Erythrocyte, cholesterol lysis

Cytotoxicity Issues. In-vivo hemolysis has been observed with parenteral administration of all of the parent CDs. In-vitro studies with human erythrocytes have demonstrated that the damaging effect of the CDs is in the order p-CD > ot-CD > y-CD. This cellular destruction has also been observed in studies with human skin fibroblasts and intestinal cells, P388 murine leukaemic cells, E. coli bacterial cells, and immortalized human corneal epithelial cells.f " Mechanistic studies suggest that CDs extract either cholesterol (p-CD and y-CD) or phospholipids (a-CD) from the cell membrane causing small pores which allow leakage and eventually lead to cell lysis. 

The effect of hemolysis on the determination of Mn-SOD levels by ELISA was examined. The lysis of erythrocytes was observed to have no effect. Because erythrocytes do not contain any Mn-SOD, the problems encountered with Cu,Zn-SOD do not occur when serum Mn-SOD levels are measured. The effects of jaundice and lipids were also examined by adding substances such as ascorbic acid, bilirubin, cholesterol, phosphatidylcholine, and triglycerides. None of the above substances affected the detection of Mn-SOD in sera. 

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