Epoxide hydrolases engineering

Rui L, 1 Cao, W Chen, KF Reardon, TK Wood (2004) Active site engineering of the epoxide hydrolase from Agrobacterium radiobacter ADI to enhance aerobic mineralization of cw-l,2-dichloroethylene in cells expressing an evolved toluene ort/io-monooxygenase. J Biol Chem 279 46810-46817. 

Lee, E.Y. and Shuler, M.L. (2007) Molecular engineering of epoxide hydrolase and its application to asymmetric and enantioconvergent hydrolysis. Biotechnology and Bioengineering, 98, 318-327. 

Bacillus subtilis, engineered to overproduce epoxide hydrolase, was used as a whole-cell biocatalyst to resolve racemic 1-benzyloxymethyl-1-methyloxirane with high (5)-selectivity. The remaining (/ )-epoxide was subsequently ring opened in situ, with inversion of stereochemistry, to obtain highly enantiomerically enriched (/ )-3-benzyloxy-2-methylpropane-l,2-diol in greater than 50 % theoretical yield (Figure 5.2). 

Rui, L., Cao, L., Chen, W., Reardon, K.F. and Wood, T.K. (2005) Protein engineering of epoxide hydrolase from Agrobacterium radiohacter ADI for enhanced activity and enantioselective production of (R)-l-phenylethane-l,2-diol. Appl. Environ. Microbiol., 71,3995-4003. 

Visser, H., de Oliveira ViUela FiUio, M., Liese, A., Weijers, C.A.G.M. and Verdoes, J.C. (2003) Construction and characterisation of a genetically engineered Escherichia coli strain for the epoxide hydrolase-catalysed kinetic resolution of epoxides. Biocatal. Biotransformation, 21,33-40. 

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