EPISKIN human skin model

EPISKIN human skin model (commercial system) Reconstructed human epidermal Cell viability (MTT-test) B.40/OECD TG 431... 

The following in vitro methods based on reconstructed human skin models are validated (by the ECVAM) for predicting skin corrosion— EPISKIN , EpiDerm , Corrositex —and irritation EPISKEM , EpiDerm , PREDISKIN , SIFT [142], Additionally, the SkinEthic was assessed and... 

EPISKIN is a three-dimensional human skin model composed of a human collagen (Types El and I) matrix, representing the dermis, covered with a film of Type IV human collagen and stratified differentiated epidermis derived from human keratinocytes. 

Flamand, N., Marrot, L., Belaidi, J.P., Bourouf L., DouriDe, E., Feltes, M. and Meunier, J.R. (2006) Development of genotoxicity test procedures with Episkin, a reconstructed human skin model towards new tools for in vitro risk assessment of dermally applied compounds Mutation Research, 606, 39-51. 

The development of a tiered testing strategy for predicting a particular kind of toxic potential, skin corrosion, based on the sequential use of a QSAR a PM based on physicochemical (pH) data and a PM based on in vitro data obtained with the EPISKIN test, a particular type of human skin model... 

Consequently, the human skin model assays (e.g. EpiDeim and EPISKIN ) and the mouse SIFT appear to be the most promising in vitro methods for skin irritation testing. However, there is a need to develop new endpoints that are more predictive of skin irritation than simple cytotoxicity determinations. 

The EpiDerm (EPI-200) skin model is mechanistically and functionally related to EPISKIN. The assay consists of normal human epidermal keratinocytes, which have been cultured in a chemically defined medium to produce a stratified, highly diEerentiated, organotypic tissue model of the human epidermis. 

In their initial studies, Tfayli et al. [33] acquired spectra from an Episkin model. This model is comprised of human adult keratinocytes which produce stratified epidermis following a 13 h culture period. Raman spectra from this model were compared with normal human skin. Significant differences were noted, particularly in spectral features arising from the 850/830 tyr Fermi doublet (which is sensitive to the H-bonding state of the -OH group [34]) and in the protein amide III region. Usable spectra were acquired to a depth of 15-20 pm. 

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