Eosine labelling

P. Yuan and D. R. Walt, pH-Dependent fluorescence of merocyanine-eosin-labelled water-soluble polymers, Macromolecules 23, 4611-4615 (1990). 

These results were extended by Tilton et a/.(n8) to adsorption of eosin-labeled BSA on polymer surfaces. They also found a component that surface diffuses, with coefficients ranging from 1.2 x 10 9 to 2.6 x 10 9cm2/s, depending on surface type. In this study, intersecting TIR laser beams rather than a focused stripe were used to define the spatial intensity variation. Surface diffusion was even noted for the most irreversibly adsorbed eosin-labeled BSA components this was evident on samples rinsed for long periods with unlabeled BSA after exposure to eosin-labeled BSA. The surface diffusion coefficient of the irreversibly bound BSA was found to be a strong function of adsorbed concentration.(n9)... 

Eosin emission characteristics depend strongly on the solvent. Specifically transfer from aqueous solution to a nonaqueous solvent shifts the emission of Eosin toward longer wavelengths and increases the emission intensity. Wang and Cheung [142] have used the fluorescence enhancement of the Eosin label to study the association of troponin I with troponin C. Similarly Skou and Esmann [143] and Helmich de Jong et al. [144] have used Eosin itself as a fluorescent probe to study the conformational changes of enzymes involved in ionic transport. 

Table 13.1. Calculated Rq Values for RET from Structural isomers of Dansyl>Labeled Ph< phatidylethanolamine (DPE) to Eosin Labeled Ethanolamlne (EPE) and from 2,6-DPE tO 2/5-DPE ...

Eosine labelling of proteins for time-resolved phosphorescence measurements... 

I 7 nwsphorescence anisotropy decay In ghosts. The anisotropy decay of eosin-labelled proteins was monitored as a function of time. The data were fitted according to eQuaUon 21. Reivinted in part with permission from Mateyoshi, E. D. and Jovin, T. M. (1991) Biochemistry, 30, 3527-3538. Copyright 1991 American Chemical Society. 

DLA Phosphorescence of Eosin-labeled Fatty Acids in Phospholipid Bilayers... 

To evaluate the bar-coded slide labels supplied by Ventana, a number of tests were done by placing bar-code-labeled slides in vials containing eosin, hematoxylin, 100% ethyl alcohol, or xylene for 5 min. All labels subjected to these tests remained firmly affixed to the slides. In a second test of the bar codes, a number 2 pencil was used to place marks onto the bar codes in an... 

Fig. 4.3. (A) Diagram of the amnion invasion assay. The invasion chamber represents a cylindrical well produced by a Teflon ring (a) to which epithelium-free amnion (b) is fastened with the aid of a viton ring (c), to face the BM side up and stromal side down. A smaller lower chamber is created by a silicone rubber ring support attached to the bottom of a 35-mm tissue culture well (d) with silicone grease, and filled with medium. The (upper) invasion chamber is placed on this support, and medium with or without additives (to be tested for invasion-blocking or stimulating ability) is added to this chamber 1 h prior to the addition of labeled cells to be tested for invasive ability. Medium is then added to the tissue culture well (d) outside these chambers to bring the fiuids inside and outside the Teflon ring to the same level (e) represents a well that includes the complete invasion chamber seeded with cells on the BM. (Reproduced from Yagel et al., 1989.) (B) (a) Human amnion. Epithelium (EP), basement membrane (BM), connective tissue stroma (ST). Haematoxylin-eosin PAS stain, (b) Denuded human amnion membrane. Basement membrane (BM), connective tissue stroma (ST), Milfipore filter (F). Haematoxylin-eosin, PAS stain. (Reproduced from Russo, 1986.)...

Sabbert, Engelbrecht and Junge " modified a chloroplast CF, -ATPase complex by labeling its y-subunit with eosin-5-maleimide (abbreviated as e5m) [Fig. 34 (A)]. It is known thateSm forms a covalent bond with Cys-322 in the y-subunit of CF,. The specific labeling by eosin was confirmed by fluorescence of the y-subunit band on SDS-PAGE viewed under U V-illumination. A sample was then prepared in which the e5m-labeled CF, is immobilized on an anion-exchange resin (Saphadex DEAE-50). 

Properly orient and embed specimen in OCT see Note 14). Quick freeze by submersing in isopentane cooled to -55 °C in the histobath. Mount specimen into the cryostat. Cut 5-pm thick sections on plus coated slides, labeled with the patients name or accession number. Fix in 10% formalin in 80% alcohol for 1 min. Rinse 10 dips in deionized water. Stain with Biomeda hematoxylin for 30 s. Rinse with deionized water. Counterstain with Eosin Y for 30 s. Dehydrate, clear in xylene, and coverslip. 

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