Eosin blue

Eosin blue Eosine blue Fluorescein, 2, 4, 5, 7 -tetrabromo-4,5,6,7-tetrachloro-, disodium salt Food dye red 104 Food red 104 Japan red 104 Phloxin B Phloxine B Red 104 Spiro [isobenzofuran-1(3H),9 -[9H] xanthen]-3-one, 2, 4, 5, 7 -tetrabromo-4,5,6,7-tetrachloro-3, 6 -dihydroxy-, disodium salt 2, 4, 5, 7 -Tetrabromo-4,5,6,7-tetrachloro-3, 6-dihydroxyspiro [isobenzofuran-1(3H),9 -[9H] xanthen]-3-one, disodium salt Classification Xanthene color Empiricai C2oH2Br4Cl40s 2Na Properties Dk. red powd. sol. 90 mg/ml in water m.w. 829.64... 

Bromophenol blue 2, 7 -DichIorofluorescein Eosin, tetrabromofluorescein Fluorescein Potassium rhodizonate, C404(0K)2 Rhodamine 6G Sodium 3-aIizarinsuIfonate Thorin Dissolve 0.1 g of the acid in 200 mL 95% ethanol. Dissolve 0.1 g of the acid in 100 mL 70% ethanol. Use 1 mL for 100 mL of initial solution. See Dichlorofluorescein. Dissolve 0.4 g of the acid in 200 mL 70% ethanol. Use 10 drops. Prepare fresh as required by dissolving 15 mg in 5 mL of water. Use 10 drops for each titration. Dissolve 0.1 g in 200 mL 70% ethanol. Prepare a 0.2% aqueous solution. Use 5 drops per 120 mL endpoint volume. Prepare a 0.025% aqueous solution. Use 5 drops. 

Testing is undertaken by several methods, including chloroform extraction and use of a sulfonphthalein dye (absorbance of yellow-colored complex using bromophenol blue and bromocresol green) or the use of eosin (sodium tetrabromofluorescein) solution in acetone and tetrachloroethane solvent. After shaking with a citric acid buffer and eosin addition, upon standing the lower layer turns pink if filmer is present. Subsequent titration with Manoxol OT (sodium dioctyl sulfosuccinate) quantifies the filmer, with loss of the pink color indicating the end point. 

Moafi et al. [143] studied the ability of titania versus zirconia to photocatalyze methylene blue and eosin yellow on polyacrylonitrile fibers. Ti02 particles ranging from 10-20 nm in size and Zr02 particles ranging from 20-40 nm in size were dispersed on the fiber surface. Photocatalytic activity of Ti02 was greater. 

Photolysis of nicotine (173) in the presence of oxygen and methylene blue with light wavelengths greater than 300 nm gave the pyrrolidone (174, 30%), nicotyrine (175, 23%) and TV-oxide (176, 7%). Under nitrogen, with eosin as photosensitizer, only nicotyrine (14%) was identified in the photodegraded mixture [112]. 

Figure 8. T (365 nm) vs. E (as indicated) for 13% DMA/PEMA/1% eosin, irradiated under N2. Note that the all-lines dose includes 50% 514 nm light, which is demonstrated in this figure to be non-actinic therefore the efficiency of the blue-light reaction is 2x greater than shown.

These polymorphs are divided into three subgroups by virtue of the staining properties of their cytoplasmic contents when treated with dye mixtures. Thus, eosinophils stain with acid dyes such as eosin and appear red in stained blood films, basophils stain with basic dyes and appear blue whilst neutrophils stain with both types of dye and their cytoplasm appears purple. The differences between the cytoplasmic contents in these cells types is much more fundamental than is suggested by these simple staining properties. The granules of basophils, neutrophils and eosinophils contain distinct molecular constituents that confer upon the cells their specialised functions during infection and inflammation. 

To obtain tissue preparations whose constituents were maintained as closely as possible to their state in vivo, the material had to be fixed, i.e. the enzymes inactivated so that cell structures were instantaneously preserved, an almost unattainable ideal. Formalin was the favored fixative, but others (e.g. picric acid), were also employed. Different methods of fixation caused sections to have different appearances. Further artifacts were introduced because of the need to dehydrate the preparations so that they could be stained by dyes, many of which were lipid-soluble organic molecules. Paraffin wax was used to impregnate the fixed, dehydrated material. The block of tissue was then sectioned, originally by hand with a cut-throat razor, and later by a mechanical microtome. The sections were stained and mounted in balsam for examination. Hematoxylin (basophilic) and eosin (acidophilic) (H and E staining) were the commonest stains, giving blue nuclei and pink cytoplasm. Eosinophils in the blood were recognized in this way. 

Giemsa stain chem A stain for hemopoietic tissue and hemoprotozoa consisting of a stock glycerol methanol solution of eosinates of Azure B and methylene blue with some excess of the basic dyes. gem-s3, stan gifblaar poison See fluoroacetic acid. gif,blar. poiz-on ... 

Nonchelating dyes include basic triphenylmethane dyes (e.g., Brilliant Green, Malachite Green, Crystal Violet), xanthene dyes (e.g., Rhodamine B, Rhodamine 6G), azine dyes (e.g., Methylene Blue), and acid dyes (e.g., Eosin, Erythrosin). These are intensely colored and when paired with an oppositely charged analyte ion lead to high sensitivities. 

Mutual precipitation of oppositely charged dye-stufifs is the basis of a number of volumetric processes of estimation thus the evaluation of tannin solutions may be accomplished with night blue or of eosine with night blue. 

Other workers have employed different sensitiser systems, e.g. duel sensitisation by a zinc porphyrin and copper phthalocyanine on TiOj, Eosin Y or tetrabro-mophenol blue on ZnO, and a ZnO/SnOj mixture with a ruthenium bipyridyl complex, to produce good energy conversion factors. 

Competitive photooxygenation of the substrate pair 1-methyl-cyclopentene (AJ/l-methylcyclohexene (A2) was studied by Kopecky and Reich.123 Using rose bengal, erythrosin, and eosin, as well as methylene blue and hematoporphyrin as sensitizers, the ratio kg1/kg2 was found to be independent of the nature of the sensitizer used, in agreement with the assumption that singlet oxygen is the oxygenating intermediate. 

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