Enzymology: mole

Under the steady-state conditions that usually apply (see p. 449), the rate of increase of product will be the same as the rate of decrease of substrate. The units of velocity are moles per liter per second (M s 1) or more traditionally in enzymology moles per liter per minute. We are interested in the instantaneous velocity, which at... 

AdapLed. from Uhleii, M., and Moles, T., 1990. Gene fusions for purpose of expression An inu oducdon. Methods in Enzymology 185 129-143. 

A unit of enzyme catalytic activity equal to the conversion of one mole of substrate per second in a specified assay system. The katal (kat) is more commonly used in clinical enzymology. One unit of enzyme activity (i.e., one micromole per minute) corresponds to 16.67 nkat. 

Figure 9-27. Buoyant densities (25°C) of native DNA s in CS2SO4 (A) and CsCl (B) gradients as a function of base composition ( , solid lines), expressed as mole percent of G + C or G + HMC, or as a function of the glucose to HMC ratio (mole %), the latter for T-even phage DNA (O, dashed lines). Denatured E. coli DNA (Den. E COLI, 0) was prepared by heating DNA (10 jag/ml) for 10 minutes at 96 to 100°C in 0.02M sodium citrate (pH = 7.8) and rapidiy chilling to 0°C. Based largely on the data of R. L. Erikson and W. Szybalski, Virology, 22 111 (1964). [From W. Szybalski, in Methods of Enzymology, Vol. 12B, Academic Press, New York, 1968, p. 330.]...

The research summarized in this manuscript was directed toward one particular problem in enzymology that is, the development of techniques which would make possible the use of cofactor-requiring enzymes in organic synthesis. The central problem in this area has been one of expense. ATP costs approximately 800/mole when purchased in mole quantities the costs of the nicotinamide cofactors range from 1500/mole (for NAU ) to 250,000/mole (for NADPH). 

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