Enzymes glucose isomerase

Immobilized enzyme Glucose isomerase Penicillin V acylase... 

Some of the industrial biocatalysts are nitrile hydralase (Nitto Chemicals), which has a productivity of 50 g acrylamide per litre per hour penicillin G amidase (Smith Kline Beechem and others), which has a productivity of 1 - 2 tonnes 6-APA per kg of the immobilized enzyme glucose isomerase (Novo Nordisk, etc.), which has a productivity of 20 tonnes of high fmctose syrup per kg of immobilized enzyme (Cheetham, 1998). Wandrey et al. (2000) have given an account of industrial biocatalysis past, present, and future. It appears that more than 100 different biotransformations are carried out in industry. In the case of isolated enzymes the cost of enzyme is expected to drop due to an efficient production with genetically engineered microorganisms or higher cells. Rozzell (1999) has discussed myths and realities... 

The third example concerns an industrially relevant enzyme, glucose isomerase (see Chapters 7,10, and 19). Table 8.6 reproduces the purification data, and Figure 8.10 shows the SDS PAGE and IEF (isoelectric focussing) diagrams. 

The most widely used immobilized enzyme process involves the use of the enzyme glucose isomerase for the conversion of glucose to fructose in com syrup (Carasik and Carroll 1983). The organism Bacillus coagu-lans has been selected for the production of glucose isomerase. The development of the immobilized cell slurry has not proceeded to the point where half-lives of the enzyme are more than 75 days. A half-life is defined as the time taken for a 50 percent decrease in activity. Such immobilized enzyme columns can be operated for periods of over three half-lives. 

The isomerization of the glucose syrup thus obtained was originally also employed in batch reactors using the Mg -containing enzyme glucose isomerase in soluble form, but rapidly evolved to the use of an immobilized enzyme... 

D-glucose, which is subsequently converted to D-fructose by the plant enzyme glucose isomerase. 

Or you can travel it via the enzyme glucose isomerase (Figure 3), and it vill take you more than a second. 

Taka-Sweet . [Solvay Enzymes] Glucose isomerase enzyme for p of fructose synq>s from glucose. 

Enzymes. Enzymes are used in many industries as catalysts. Microorganisms are the favored source for industrial enzymes. Seventy percent of these enzymes are made from Bacillus bacteria via fermentation. Most commercial microbial enzymes are hydrolases, which break down different organic molecules such as proteins and lipids. The enzyme glucose isomerase is important in the production of fructose syrups from corn and is widely used in the food industry. 

The commercial enzyme (glucose isomerase) is used to convert high-glucose com symps into high-fructose com symps that are commonly used as a sweetening agent in soft drinks. This enzyme is actually a xylose isomerase that can catalyze the isomerizations of both D-glucose and D-xylose. 

The enzyme glucose isomerase converts glucose into a mixture of glucose and fructose (1 1) mixture. Fructose is preferentially hydrogenated from this equilibrium mixture into mannitol. Elevated temperature (70°C) and high hydrogen pressure (70 bar) do not have a deteriorating influence on the enzyme s performance. 

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