Enzymes glucoamylase

Multienzyme Electrodes. Coupling the reactions of two or more immobilized enzymes increases the number of analytes that can be measured. An electro-inactive component can be converted by an enzyme to a substrate that is subsequentiy converted by a second enzyme to form a detectable end product (57). For example, a maltose [69-79-4] sensor uses the enzymes glucoamylase and glucose oxidase, which convert... 

Figure 2-29 Structure of the a,a barrel of a fungal enzyme glucoamylase. (A) side view (stereoscopic) (B) top view. The active site, which cleaves glucose units from the ends of starch chains, is in the depression in the center of the barrel. Here it is occupied by an inhibitor. See Aleshin et al.192 Courtesy of Alexander Aleshin.

Getting ethanol from corn consists of five steps grain grinding, mash cooking, starch liquefaction, saccharification and fermentation, followed by ethanol separation and dehydration. Before fermentation, the starch has to be converted to soluble dextrin by liquefaction in the presence of specific enzymes, such as a-amylase. This process takes place at temperatures above 100 °C at suitable pH and residence time. Then, another enzyme glucoamylase is added that transforms the... 

Publications giving the details of the actual development work in establishing industrial microbial enzyme processes are non-existent. It is, therefore, of interest to describe the development of a commercial enzyme process for one of the most important industrial enzymes, glucoamylase. Although there are many patents and publications on glucoamylase, nowhere has there previously been an attempt to gather together the available published and unpublished non-confidential information into a coherent report. 

Maltose is also determined through the coupling of two enzymes glucoamylase and glucose oxidase [165,166] ... 

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