Enzyme Assays Glucoamylase Activity

Enzymes, such as glucoamylase and 3-glucosidase, which differ in their secondary and tertiary stmctures, were adsorbed on SPEB. ° The activity of the enzymes was analyzed in terms of the Michaelis-Menten parameters Km and fecat. This study demonstrated that the activity of the enzymes was preserved despite the dense packing of proteins within the bmsh (Figure 31). Recently, it was shown that ITC is a useful tool to study the activity of immobilized enzymes. This technique allows studies of the enzymatic activity in turbid media, such as in concentrated suspensions of the SPEB. Moreover, ITC does not demand chromophores or fluorophores as photometric assays do. Thus, this method is very versatile because unlabeled substrates can be used. The results from ITC were in agreement with a typical photometric study, and showed that the activity of (1-glucosidase adsorbed on the SPEB is retained. 

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