Enzymes diagnostic uses

Any substance that reduces the velocity of an enzyme caialyzed reaction can be considered to be an "inhibitor. The inhibition of enzyme activity is one of the major regulatory devices of living cells, and one of the most important diagnostic procedures of the enzymologisL Inhibition studies often tell us something about the specificity of an enzyme, the physical and chemical architecture of the active site, and the kinetic mechanism of the reaction. In OUT everyday life, enzyme inhibitors can be found masquerading as drugs, antibiotics, preservatives, poisons, and toxins. In this section we examine a few simple types of enzyme inhibitors. 

In a realistic system there are many other motions present, so there is no guarantee that the effect of the promoting vibration will not be masked by other interactions that are present in an enzyme. We need a diagnostic that when we perform a computer simulation of the dynamics of an enzyme, will allow us to identify whether a promoting vibration is present. 

Chamoles, N. A. Determination of the activity of lysosomal enzymes in dried body fluid or tissue sample for diagnostic applications. U.S. Pat. Appl. Publ. US 2004166554, 2004 Chem. Abstr. 2004, 141, 202270. 

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