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Enzyme fragment complementation EFC

There are cAMP assays that can be measured using a variety of techniques [35], including HTRF (CisBio) [36], bioluminescence (cAMP-Glo, Promega) [37], TR-FRET (CisBio) [38], LANCE [39], Alphascreen (Perkin Elmer) [40] and Enzyme Fragment Complementation (EFC) [41] (Hithunter, DiscoverX). This review will focus on the LANCE, the TR-FRET and ECF assays as they are the most used in our laboratories. [Pg.45]

FIGURE 4.1 Assays commonly used in GPCR research. SPA = scintillation proximity assay FP = fluorescence polarization TR-FRET = time-resolved fluorescence resonance energy transfer FCS = fluorescence correlation spectroscopy SeAP = secreted alkaline phosphate TF = transcription factor EFC = enzyme fragment complementation DMR = dynamic mass redistribution CDS = cellular dielectric spectroscopy. [Pg.61]

See other pages where Enzyme fragment complementation EFC is mentioned: [Pg.265]    [Pg.66]    [Pg.648]    [Pg.648]    [Pg.168]    [Pg.499]    [Pg.265]    [Pg.66]    [Pg.648]    [Pg.648]    [Pg.168]    [Pg.499]    [Pg.88]    [Pg.66]   
See also in sourсe #XX -- [ Pg.648 ]



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