Enzyme Assays Based on Mass Spectrometry

Continuous-flow Enzyme Assays Based on Mass Spectrometry 195 4 4 4... [Pg.195]

Irth H Continuous-flow systems For ligand binding and enzyme inhibition assays based on mass spectrometry, chapter 5. [Pg.182]

Continuous-flow Systems for Ligand Binding and Enzyme Inhibition Assays Based on Mass Spectrometry... [Pg.185]

Niessen, H. Irth On-line coupling of high-performance liquid chromatography to a continuous-flow enzyme assay based on electrospray ionization mass spectrometry. Anal Chem 2004, 76, 3155-3161. [Pg.214]

Additionally it has been our experience that mass spectrometry as a routine detection/identification technique for bacteria is not well received by microbiologists and clinicians who prefer less expensive, less complicated approaches to bacterial typing and identification, such as methods based on polymerase chain reaction (PCR) and enzyme-linked immunosorbent assays (ELISA). For that reason we have adapted our MS approach to serve as a means of biomarker discovery that feeds candidate proteins or leads into development as PCR targets or other immunoassay techniques. [Pg.205]

Most of the analytical methods for the analysis of pesticides in food are based on instrumental approaches based on chromatography coupled to mass spectrometry. However, a great effort of development has been paid to develop rapid screening methods based on biological methods, such as, enzyme linked immunosorbent assays (ELISA). [Pg.22]

Modern tHcy methods include enzyme immunoassays and chromatographic-based methods. In practice, immunoassays " are most often used for routine purposes (e.g., fluorescence polarization immunoassay IFPIA] as run on Abbott s IMx and AxSYM platforms) Chromatographic assays include amino acid analysis high-performance liquid chromatography (HPLC) with ultraviolet, fluorescence, or electrochemical detection ° " capillary electrophoresis with fluorescence detection gas chromatography-mass spectrometry (GC-MS) and liquid chromatography with tandem MS (MS-MS). [Pg.968]

See also Electrophoresis Two-Dimensional Gels Nucleic Acids. Enzymes Enzyme-Based Assays. Flow Injection Analysis Principles. Fluorescence Quantitative Analysis. Lab-on-a-Chip Technologies. Mass Spectrometry Matrix-Assisted Laser Desorption/loniza-tion Time-of-Flight. Microelectrodes. Microscopy Overview. pH. Process Analysis Overview Chromatography Electroanalytical Techniques Sensors Acoustic Emission Maintenance, Reliability, and Training. Proteins Overview. Proteomics. Purines, Pyrimidines, and Nucleotides. Sensors Oven/iew. Spectrophotometry Overview. [Pg.3908]