Enzymatic synthesis initial reaction rate

The application of SCF as reaction media for enzymatic synthesis has several advantages, such as the higher initial reaction rates, higher conversion, possible separation of products from unreacted substrates, over solvent-free, or solvent systems (where either water or organic solvents are used). Owing to the lower mass-transfer limitations and mild (temperature) reaction conditions, at first the reactions which were performed in non-aqueous systems will be transposed to supercritical media. An additional benefit of using SCFs as... 

The present work reports results and observations on the enzymatic synthesis of oleyl oleate (which is a synthetic analogue of jojoba oil) in supercritical carbon dioxide. Special stress was laid on the comparison between batch and continuous systems for the above mentioned synthesis. Influence of different reaction parameters on the reaction yield and initial reaction rates was studied. 

Figure 2.9 Enzymatic synthesis of jj-nitrobenzyl-D-glucopyranoside (pNBG) by reverse hydrolysis in a monophasic dioxane-water system. The reaction was performed in 1.0 mL of dioxane-buffer medium (Na2HP04-KH2PO4, 70 mM, pH 6.0) by shaking a mixture of 0.25 mmol glucose, 1.0 mmol pNBA and 5 mg of enzyme powder at 50 °C and 160 rpm. Symbols ( ) initial rate ( ) final conversion.

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