Enzymatic reaction systems

One of the most frequently studied enzymatic reaction systems involves the use of glucose oxidase ... 

ENZDYN - Dynamic Diffusion with Enzymatic Reaction System... 

Calculated for a simplified model system [100] at the MP2/6-31+G(d)//HF/3-21G level. The value in parenthesis was calculated at the MP2/6-31+G(d)//B3LYP/6-31G(d) level Calculated for the real enzymatic reaction system by using the QM/MM method at the MP2/6-31+G(d) Amber level with the geometries optimized at the HF/3-21G Amber level... 

The difference between the QM/MM-calculated energy barriers for the rate-determining steps of the two enzymatic reaction systems is consistent with the experimental observation that the fccat value (1.6 x 10" s ) [124] for AChE-catalyzed hydrolysis of ACh was about 150-fold larger than that (fccat = 1.07 X 10 s ) [97] for BChE-catalyzed hydrolysis of (+)-cocaine. Based on the widely used classical transition-state theory (CTST) [125], the experimental fccat difference of 150-fold suggests an energy barrier difference of 3.0 kcal/mol when T = 298.15 K, which is in good agreement with the calculated barrier difference of 3.7 kcal/mol [113]. 

Overall, the book presents a relatively new and focused topic on supercritical fluid technology In enzymatic reaction system[s]. The writing style of the book has a good flow of information and [is] easy to understand. ... 

Select an enzymatic reaction system from the human body. Identify the active site, and show the relevant heat- and mass-transport process across the enzyme peripherals as well as the rate-controlling step. Discuss the relative roles of chemical conversion and transport rates and determine the order of magnitude of the overall rate of conversion. 

Xue, R. and Woodley, ). M. (2012) Process technology for multi-enzymatic reaction systems. Bioresour. Technol., 115, 183-195. 

Bird, R.B., W.E. Stewart, and E.N. Lightfoot, 2002. Transport Phenomena, 2nd Edition, Wiley, New York. Brodkey,R.S. andH.C. Hershey, 1988. Transport Phenomena A Unified Approach. McGraw-Hill, New York. Bruns D., J. Bailey, and D. Luss, 1973, Steady state multiplicity and stabihty of enzymatic reaction systems. Biotech Bioeng, 15,1131. 

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