Enzymatic Properties of LSADH

ACDH MSN KFNI QvQHT mAGS rnKST LLlB 3QE /s 

QT DQDAQI DG A I ERDMl PAS iQsQl E K I RAEI QtvesdQi nlQqsai ke QssyQacv egiQkvead 

Until now, LSADH has been found to catalyze the reduction of more than 50 kinds of ketones, indicating a quite broad substrate spectrum. 

Engineering of PAR for Increasing Activity in 2-Propanol/Water Medium 

We showed the high ability of PAR to produce various chiral alcohols coupled with NADH self-regeneration in the presence of IPA as a hydrogen donor in the previous section. IPA can also profitably act as a solvent for the PAR substrates that cannot dissolve in aqueous media. However, at high concentrations of IPA, the efficiency of substrate conversion by PAR clearly fell. Consequently, the conversion yields with high substrate concentrations were relatively low and insufficient for industrial application. 

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