Enzymatic hydrolysis mechanistic models

Catalysis, enzymatic, physical organic model systems and the problem of, 11,1 Catalysis, general base and nucleophilic, of ester hydrolysis and related reactions, 5,237 Catalysis, micellar, in organic reactions kinetic and mechanistic implications, 8,271 Catalysis, phase-transfer by quaternary ammonium salts, 15,267 Catalytic antibodies, 31,249... 

Fan, L. T. and Y.-H. Lee, "Kinetic Studies of Enzymatic Hydrolysis of Insoluble Cellulose Derivation of a Mechanistic Kinetic Model," Biotech. Bioeng. 25 (1983) 2707-2733. 

Okazaki, M. and M. Moo-Young, "Kinetics of Enzymatic Hydrolysis of Cellulose Analytical Description of a Mechanistic Model," Biotechnol. Bioeng. 20 (1978) 637-663. 

There have been numerous empirical and semi-empirical models proposed for the enzymatic hydrolysis of cellulose that include inhibition terms (Andric et al., 2010a,b). The use of these models can be helpful with process design and economic calculations where it is necessary to account for the limitations imposed by inhibition. More rigorous mechanistic models for enzymatic hydrolysis have also included terms for product inhibition, as described in section 4.4. 

FIGURE 4 J Qualitative comparison of mechanistic model predictions (top row, from Griggs et al. (2012b)) with experimental results (bottom row, from Srisodsuk et al. (1998)) for the changing DP distribution of cellulose during enzymatic hydrolysis by EG, and CBH,. The left column compares resnlts for bacterial microcrystalline cellulose (BMCC) with a relatively low initial DP, and the right column compares results for cotton linter with a relatively high initial DP. For a color version, see the color plate section.)... 

Levine SE, Fox JM, Blanch HW, Clark DS. (2010). A mechanistic model of the enzymatic hydrolysis of cellulose. Biotechnol Bioeng, 107(1), 37-51. 

Okazaki M, Mooyoung M. (1978). Kinetics of enzymatic-hydrolysis of cellulose analytical description of a mechanistic model. Biotechnol Bioeng, 20(5), 637-663. 

Enzymatic action can be defined on three levels operational kinetics, molecular architecture, and chemical mechanism. Operational kinetic data have given indirect information about cellulolytic enzyme mode of action along with important information useful for modeling cellulose hydrolysis by specific cellulolytic enzyme systems. These data are based on measurement of initial rates of enzyme hydrolysis with respect to purified celluloses and their water soluble derivatives over a range of concentrations of both substrate and products. The resulting kinetic patterns facilitate definition of the enzyme s mode of action, kinetic equations, and concentration based binding constants. Since these enable the enzymes action to be defined with little direct knowledge of its mechanistic basis, the rate equations obtained are referred to as operational kinetics. The rate patterns have enabled mechanisms to be inferred, and these have often coincided with more direct observations of the enzyme s action on a molecular level [2-4]. 

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