Enzymatic degradation and detoxification of diethyl phthalate by

Enzymatic Degradation and Detoxification of Diethyl Phthalate by Fusarium oxysporum f. sp.pisi Cutinase 

Cutinase is a hydrolytic enzyme that degrades cutin, the cuticular polymer of higher plants [4], Unlike the oflier lipolytic enzymes, such lipases and esterases, cutinase does not require interfacial activation for substrate binding and activity. Cutinases have been largely exploited for esterification and transesterification in chemical synthesis [5] and have also been applied in laundry or dishwashing detergent [6]. 

The purified fungal cutinase fiom F. oxysporum f. sp. pisi was kindly provided by Prof. C.M.J. Sagt in Utrecht University. The commercial product of esterase firom Candida cylindracea was purchased fi om Boehringer Mannheim (Germany). The enzymatic degradation of DEP 

The estimation of the initial degradation constants, kcio and kEioo, for cutinase (10 mg f ) and esterase (100 mg l ), respectively, demonstrated that the initial DEP degradation by cutinase seemed to proceed almost 2.6 times faster. 

DNA Membrane Oxidative Protein damage damage damage damage 

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