Environmental chambers light sources

Sherer Environmental Chambers models CEL-512-37 and CEL-34-14 were freshly outfitted with incandescent (93W) and cool white VHO fluorescent bulbs. Eleven plants from each of the three sources were divided between the two chambers. Plant-top light-intensity varied from 2,800-3,300 ft-c, depending on plant height and the chamber involved. Controls were set for maximum relative humidity (measurements varied between 50 and 100%). Temperature was set at 22°C day (16H) and 17°C night (8 H). Plants were grown under these conditions for 12 wk. Beginning 24 Jan 1980, daylength was decreased from 16 to 11 h over a 4 wk period. 

Figure 21 Environmental chambers (A) temperature, (B) humidity and (C) light chambers. Source Courtesy of Termaks.

The ApoE (— / —) mice in the current study were placed on a high fat/ cholesterol (1-2%) diet for 3 months until sacrifice. We employed Beamline 1.4.3 at the Advanced Light Source (ALS) synchrotron at LBNL (http // infrared.als.lbl.gov/) as our mid-IR source. The synchrotron IR beam has brightness in the range of approximately 10 to 7x10 photons/sec-mm mrad-0.1%BW when focused to a 10 pm spot. Each aortic specimen was placed luminal side up beneath an IR-transparent ZnSe window (International Crystal Labs, NJ) inside a custom-built environmental chamber where the temperature was kept at 5 °C to maintain the freshness of the specimens during reflectivity measurements. All FTIR measurements were recorded in the standard reflection mode in the mid-IR 4000 to 650 cm region, and consisted of 128 co-added spectra at a spectral resolution of 4cm ... 

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