Enoyl-coenzyme A

Uchicda, Y., Izai, K., Orii, T., Hashimoto, T. (1992). Novel fatty acid p-oxidation enzymes in rat liver mitochondria. II. Purification and properties of enoyl-coenzyme A (CoA) hydratase/3-hy-droxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase trifunctional protein. J. Biol. Chem. 267, 1034-1041. 

Kiang, H., Luo, L., Taylor, K. L., and Dunaway-Maariano, D. (2000) Interexchange of catalytic activity within the 2-enoyl-coenzyme A hydratase/isomerase super family based on a common active site template,... 

D Ordine, R.L., Bahnson, B.J., Tonge, P.J. Anderson, V.E. (1994) Biochemistry 33, 14733-14742. Enoyl-coenzyme A hydratase-catalyzed exchange of the a -proton of coenzyme A thiol esters a model for an enolized intermediate in the enzyme-catalyzed elimination ... 

Yang, S.-Y, He, X.-Y. Schulz, H. (1995) Biochemistry 34, 6441-6447. Glutamate 139 of the large a-subunit is the catalytic base in the dehydration of both D- and L-3-hydroxyacyl-coenzyme A but not in the isomerization of A , A -enoyl coenzyme A catalyzed by the multienzyme complex of fatty acid oxidation from Escherichia coli. 

Engel, C.K., Mathieu, M., Zeelen, IP., Hiltunen, J.K. Wierenga, R.K. (19%)EMBOJ. 15, 5135-5145. Crystal structure of enoyl-coenzyme A (CoA) hydratase at 2.5 angstrom resolution a spiral fold defines the CoA-binding pocket. 

Yang, S.-Y. Elzinga, M. (1993)7. Biol. Ghem. 268, 6588-6592. Association of both enoyl coenzyme A hydratase and 3-hydroxyacyl coenzyme A epimerase with an active site on the amino-terminal domain of the multifunctional fatty acid oxidation protein from Escherichia coli. 

Waterson, R.M. Hill, R.L. (1972) J. Biol Chem. 247, 5258-5265. Enoyl coenzyme A hydratase (cro-tonase). Catalytic properties of crotonase and its possible regulatory role in fatty acid oxidation. 

Fukui T, Doi Y (1998) Expression and characterization of (/f)-specdlic enoyl coenzyme A hydiatase involved in polyhydroxyalkanoate biosynthesis by Aeromonas caviae. J Bacteriol 180 667-673 Fukui T, Abe H, Doi Y (2002) Engineering of Ralstonia euttopha for production of poly(3-hydroxy-butyrate-co-3-hydroxyhexanoate) from fructose and solid-state properties of the copolymer. Biomacromolecules 3 618-624... 

Coenzyme A thioesters can also promote nucleophilic attack at the j8-carbon in o(,j8-enoyl derivatives. In these cases an electrophilic centre is stabilized at the j8-carbon by resonance with the carbonyl system. This could be particularly favoured by hydrogen bonding or protonation of the carbonyl oxygen by an enzyme. An example is the enoyl coenzyme A hydratase reaction of fatty acid degradation. 

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