Energetics, of proteins

C. The Unrecognized Role of the Ribosome in the Energetics of Protein Conformations... 

T. Sengupta, L. Razumovsky, and S. Damodaran Energetics of Protein-Interface Interactions and its Effect on Protein Adsorption. Langmuir 15, 6991 (1999). 

STRUCTURES AND ENERGETICS OF PROTEINS AND THEIR ACTIVE SITES... 

Sengupta, T., Razumovsky, L., Damodaran, S. (1999). Energetics of protein-interface interactions and its effect on protein adsorption. Langmuir, 15, 6991-7001. 

Computer simulations are regularly performed of the energetics of protein structure and their interactions with ligands using potential energy functions for the forces described so far. The energy functions are of necessity simplifications, but they are calibrated on experimental data. A minimalist model uses equation 11.3.15... 

The energetics of protein association can be studied by a variety of experimental techniques,17 each of which permits measurements of equilibrium or kinetic values in a certain range. Widely used techniques include isothermal titration calorimetry, surface plasmon resonance measurement, stopped flow kinetics, optical spectroscopy, MS, and analytical ultracentrifugation. The techniques differ in their requirements (e.g., amount of protein, labeling with fluorophores, attachment to sensor surfaces, and the environment provided by the experimental set up) and therefore in their applicability to individual cases. Different techniques can also give quite different values for what might be expected to be the same quantity. For example, association rates measured by surface plasmon resonance, with one protein immobilized on a surface, are usually different from those measured for the two proteins in solution and under otherwise similar conditions. 

Schreiber G, Fersht AR (1995) Energetics of protein-protein interactions analysis of the barnase-barstar interface by single mutations and double mutant cycles, J Mol Biol, 248 -178 1X9... 

Chemistry and Chemical Reactivity of Proteins, Matthew Francis Energetics of Protein Folding, Robert Baldwin NMR to Study Proteins, Angela Gronenborn Physical Chemistry in Biology, Allan Cooper... 

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