Energetics of Ligand Binding to Proteins

The subtilisin mutants described here illustrate the power of protein engineering as a tool to allow us to identify the specific roles of side chains in the catalytic mechanisms of enzymes. In Chapter 17 we shall discuss the utility of protein engineering in other contexts, such as design of novel proteins and the elucidation of the energetics of ligand binding to proteins. 

Hagler, Structure and energetics of ligand binding to proteins Escherichia coli dihydrofolate reductase-trimethoprim, a drug-receptor system, Proteins 4 31 (1988). 

Energetics of Ligand Binding to Proteins Gregorio Weber... 

Fig. 2 Factors controlling diatomic ligand binding to hemoglobins. The Fe atom is displaced from the mean plane of the heme m deoxyMb (left) and moves into the heme plane on binding O2 (center) or CO (right) Through the proximal histidine the protein may influence the Fe displacement oq and thus the energetic barrier to ligand binding. The shape and polarity of...

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