Endoglucanases Trichoderma reesei

Cellulomonas fimi Endoglucanase A (i7) Microbispora bispora Endoglucanase I (5) Streptomyces sp. Cellulase A (72) Trichoderma reesei Cellobiohydrolase II (75)... 

Trichoderma reesei endoglucanase Egni PGAT2lT2STRP2SGPT4RA(TS)2S2TP2TS2 35... 

The core-enzymes, prepared in our laboratory, and containing the active centers, were successfully crystallized (Dr. Jones, Uppsala, communicated) and tertiary structures will be described in the near future. Chemical modification studies on these enzymes are currently being undertaken in our laboratory identification of important catalytic residues and location of the active centers will lead to more functional information on these enzymes. Other cellulases such as some endoglucanases from Clostridium thermocel-lum (EG A, EG B, EG D) (10) and EngA and Exg from Cellulomonas fimi (19) also contain sequences of conserved, terminally located and sometimes reiterated, amino acids. Some of these sequences are preceded by proline-serine rich domains. Thus, a bistructural-bifunctional organization seems to be a rather common feature among cellulases, at least for EngA and Exg from C. fimi and the enzymes from Trichoderma reesei. 

Figure 1. Fractionation of proteins in the culture filtrate of Trichoderma reesei according to their pi values Xyl, xylanase Ara, arabinosidase AE, acetyl esterase / X, /3-xylosidase aG, a-glucuronidase / G, / -glucosidase CBH, cellobiohydrolase EG, endoglucanase. Chromatofocusing was performed in a PBE-94 anion exchange resin (Pharmacia) with a pH-gradient created by ampholyte buffers (Pharmacia). Solid line, A dotted line, pH. (Reproduced with permission from ref. 24. Copyright 1988.)...

Claeyssens, M., van Tilbeurgh, H., Kamerling, J. P., Berg, J., Vrsanska, M., and Biely, P. 1990. Studies of the cellulolytic system of the filamentous fungus Trichoderma reesei QM 9414 substrate specificity and transfer activity of endoglucanase I. Biochem. 1,270,251-256. 

Kleman-Leyer, K. M., Siika-Aho, M., Teeri, T. T., and Kirk, T. K. 1996. The Cellulases Endoglucanase I and Cellobiohydrolase II of Trichoderma Reesei act synergistically to solubilize native cotton cellulose but not to decrease its moleculare size. Appl. Environ. Microbiol., 62,2883-2887. 

Medve, J., Karlsson, J., Lee, D., and Tjerneld, F. 1998. Hydrolysis of microcrystalline cellulose by cellobiohydrolase I and endoglucanase II from Trichoderma reesei Adsorption, sugar production pattern and synergism of the enzymes. Biotech. Bioeng.,59, 621-634. 

Park, C., Chang, C., and Ryu, D. 2000. Expression and high-level secretion of Trichoderma reesei endoglucanase I in Yarrowia lipolytica. Appl. Biochem. Biotechnol., 87,1-15. 

Penttila, M., Lehtovaara, R, Nevalainen, H., Bhikhabhai, R., and Knowles, J. 1986. Homology between cellulase genes of Trichoderma reesei complete nucleotide sequence of the endoglucanase I gene. Gene, 45, 253-263. 

Penttila, M., andre, L., Saloheimo, M., Lehtovaara, E, and Knowles, J. 1987. Expression of two Trichoderma reesei endoglucanases in the yeast Saccharomyces cerevi-siae. Yeast., 3,175-185. 

Lemos, M. A., Teixeira, J. A., Domingues, M. R. M., Mota, M., and Gama, F. M., The enhancement of the cellulolytic activity of cellobiohydrolase I and endoglucanase by the addition of cellulose binding domains derived from Trichoderma reesei. Enzyme Microbial Technol 2003, 32, (1), 35—40. 

Ladisch MR, Gong C-S, Tsao GT (1980) Cellobiose hydrolysis by endoglucanase (glucan glucanohydrolase) from Trichoderma reesei-. kinetics and mechanism. Biotechnology and Bioengineering 22 1107-1126... 

Ladisch MR, Lin KW, Voloch M, Tsao GT (1983) Process considerations in the enzymatic hydrolysis of biomass. Enzyme and Microbial Technology 5 82-102 Kleywegt GJ, Zou J-Y, Divine, Dales C, Gideon J, Sinning 1, Stahlberg J, Reinikainen T, Srisodsuk M, Terri TT, Jones TA (1997) The crystal structure of the catalytic core domain of endoglucanase I from Trichoderma reesei at 3.6 A resolution, and a comparison with related enzymes. Journal of Molecular Biology 272 383-397... 

M. Srisodsuk, J. Lehtio, M. Linder, E. Margolles-Clark, T. Reinikainen, and T. T. Teeri, Trichoderma reesei cellobiohydrolase I with an endoglucanase... 

Srisodsuk, M., Kleman-Leyer, K., Keranen, S., Kirk, T.K., Teeri, T.T., 1998. Modes of action on cotton and bacterial cellulose of a homologous endoglucanase-exoglucanase pair from Trichoderma reesei. Eur. J. Biochem. 251, 885-892. 

Kubicek CP (1987) Involvement of a coniditil endogluctmase tmd a plasma-membrane-bound beta-glucosidase in the induction of endoglucanase synthesis by cellulose in Trichoderma reesei. J Gen Microbiol 133 1481-1487... 

Allmaier, G. Schmid, E. R. Hagshel, K. Kubicek, C. P. Karas, M. Hillenkamp, F. Strategy for the characterization of the glycoprotein endoglucanase 1 isolated from Trichoderma reesei Combination of plasma desorption and UV laser desorption time-of-flight mass spectrometry. Anal. Chim. Acta 1990,241, 321-327. 

Srisodsuk, M Lehtio, JML Msrgolles-Clark, E Reinikainen, T Teeri, TT. Trichoderma reesei cellobiohydrolase I with an endoglucanase cellulose-binding domain action on bacterial microcrystalline cellulose. Journal of Bacteriology, 1997, 57, 49-57. 

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