Encoded secondary amine binary tags

Scheme 3.2. Secondary amine tag binary encoding method.

Ni, Z.-J., Maclean, D., Holmes, C.P., and Gallop, M.M. Encoded Combinatorial Chemistry Binary Coding Using Chemically Robust Secondary Amine Tags. In Methods in Enzymology, vol. 267, Combinatorial Chemistry. Abelson, J.N. (ed.). Academic Press San Diego pp. 261-272 (1996). 

Z. J. Ni, D. Maclean, C. P. Holmes, M. A. Gallop, Encoded combinatorial chemistry binary coding using chemically robust secondary amine tags. Methods Enzymol 1996, 267, 261-272. 

Identification of active library components is done by micro sequencing (peptide libraries) or by incorporation and recognition of the tag associated with each bead (nonpeptide libraries). An example of this latter approach, known as encoded combinatorial synthesis [19], may use the dialkylamine tagging system shown in Fig. 7. The amine group of the tag addition site is acylated with iminodi-acetic anhydride to yield a pentafluorophenyl ester derivative. The active ester is reacted with a binary mixture of secondary alkylamines, leading to a tag polymer. 

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