Embryonic chick cartilage

Hatanaka, H., and Egami, F., 1976, Sulfate incorporation from ascorbate 2-sulfate into chondroitin sulfate by embryonic chick cartilage epiphyses, J. Biochem. 80 1215-1221. 

Embryonic chick cartilage EmbrA onic chick cartilage Embr> onic chick c-anilage ... 

Caplan AI, Rosenberg MJ (1975) Interrelationship between poly(ADP-ribose) synthesis, intracellular NAD levels and muscle or cartilage differentiation from mesodermal cells of embryonic chick limb. Proc Natl Acad Sci USA 72 1852-1857... 

Searls RL, Janners M (1969) The stabilization of cartilage properties in the cartilage forming region of the embryonic chick limb. J Exp Zool 170 365-376... 

Dingle, J.T., Lucy, J.A., Fell, H.B. Studies on the mode of action of excess of vitamin A. I. Effect of excess of vitamin A on the metabolism and composition of embryonic chick-limb cartilage grown in organ culture. Biochem. J. 79, 497-500 (1961)... 

Further evidence that C-25 and C-l hydroxylation are the activation steps of vitamin D3, and that C-24/C-23 and C-23/C-26-lactone metabolic conversions do not produce physiologically important products is with the use of side-chain fluoridated analogues of 25-OH-D3 [87, 88], Studies with these analogues were prompted by assertions that 24-hydroxylated or lactone metabolites are involved or required for such biological actions as mineralization of bone [89], suppression of parathyroid hormone secretion [90], cartilage metabolism [91], and embryonic development in the chick [92]. It is well established that plasma 24,25-(OH)2D3 concentrations (2-5 ng/ml) are approximately 50 times greater than those of l,25-(OH)2D3. Even so,... 

Various studies (Jll) of the incorporation of D-[6- C] ucose, L-[i C]lysine, and [ °S]sulfate into the chondroitin sulfate proteoglycan of chick embryonic cartilage show that the carbohydrate and protein moieties are synthesized at different relative rates. Furdier approaches to the order of biosynthesis of the proteoglycan moieties have been made (e.g., B17, M13, M16, 06 see also Section 5, p. 36) by a comparison of their rates of formation in the presence of a specific inhibitor of the biosynthesis of one of them, and all the observations accord with protein synthesis preceding polysaccharide elaboration. 

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