Embryo cells tissue dissociation

N-CAM the prototype adhesion molecule of the immunoglobulin superfamily N-CAM was first identified in chick embryo nervous tissue using an antibody that specifically inhibited the re-aggregation of dissociated nerve cells [56,57]. Subsequently, the antibodies were shown to alter the normal patterns of differentiation in the chick embryo, to inhibit the bundling of neurites into nerve fascicules and to prevent the... 

The Drosophila neuromusculature has been subject to extensive physiological investigation for more than 20 years (Jan and Jan 1976a,b Jan et al. 1997). It has been the primary tissue for the study of ionic conductances, synaptic transmission, and the output of central pattern generation. In all cases, recording has focused on the somatic muscles and the output onto these muscles by the glutamatergic NMJ. Discussed here are neuromuscular cultures derived from dissociated cells and whole-embryo culture, and recording techniques from the embryonic, larval, and adult neuromusculature in situ. 

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