Ellipsometry fibrinogen

Welin-Klinstroem et al used a null ellipsometer equipped with an automatic sample scanning device for studies of adsorption and desorption of fibrinogen and IgG at the liquid/solid interface on surface wettability gradients on silicon wafers. To follow the processes along the wettability gradient, off-null ellipsometry was used. The kinetics of adsorption and nonionic-surfactant-induced desorption varied considerably between fibrinogen and IgG. In the hydrophilic region, veiy little protein desorption was seen when a nonionic surfactant was used. 

Very little of the research that has been done on these proteins has involved the use of electrochemical techniques. Instead, ellipsometry, FTIR/ATR spectroscopy, radioactive labeling, and photon correlation spectroscopy have been used. Many of the studies have been directed toward the development of biocompatible polymer surfaces. The first event that takes place after contact of blood or plasma with an artificial surface is the rapid adsorption of proteins from the blood onto the material surface. It is generally assumed that all subsequent events, such as platelet adhesion and surface activation of blood coagulation, are determined by the composition and structure of the initially adsorbed protein layer. It is known from in vitro experiments that the adhesion of platelets is promoted when fibrinogen has been adsorbed on a material surface and that platelet adhesion is reduced when preadsorbed albumin is present on the surface. In a study of the adsorption behavior of three of the more abundant... 

Adsorption of biologically important materials, such as fibrinogen from plasma proteins and barbiturates, " and organic molecules including corrosion inhibitors have been studied, often as functions of potential of the substrate, by ellipsometry in situ. Jovancicevic, Yang, and Bockris used time-resolved ellipsometry to measure the thickness, refractive index, and extinction coefficient of the adsorption layer of l-octyne-3-ol on iron as functions of time. They showed that the layer thickness increased stepwise, due to change of the adsorption configuration from flat... 

Yu Y, Jin G (2005) Influence of electrostatic interaction on fibrinogen adsorption on gold studied by imaging ellipsometry combined with electrochemical methods. J Colloid Interface Sci 283 477 81... 

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