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ELISA enzyme-linked formulations

A partially purified Bacillus thurlnglensis var. israelensls (Bti) 6-endotoxin was used to Immunize rabbits. The antisera obtained have an improved specificity towards the mosquito larvacidal activity of the toxin, as opposed to antiserum raised when the whole crystal was used as immunogen. Using a two step/indirect ELISA (enzyme linked immunosorbent assay) procedure developed in our laboratory, fourteen experimental formulations were tested, and the results were compared with bioassays. An average of 69.1 international units 20% c.v. was found to associate with each ug of toxin detected by the ELISA. Our data indicate that when toxin specific antisera are available, Immunoassays can be used to predict the biological activity of Bti samples with reasonable accuracy. [Pg.320]

Braun, A. and J. Alsenz (1997). Development and use of enzyme-linked immunosorbent assays (ELISA) for the detection of protein aggregates in interferon-alpha (IFN-alpha) formulations. Pharm Res 14(10) 1394-1400. [Pg.302]


See other pages where ELISA enzyme-linked formulations is mentioned: [Pg.143]    [Pg.30]    [Pg.707]    [Pg.333]    [Pg.593]    [Pg.81]   
See also in sourсe #XX -- [ Pg.298 , Pg.299 ]




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