Electrophoretic patterns, serum alkaline

Figure 21-5 A, Poiyacrylam id e-gel electrophoresis of bone and liver alkaline phosphatases in human serum. Left, Mixture of two sera containing, respectively, entirely bone phosphatase and entirely liver phosphatase. Right, Mixture of the same two sera after each has been treated with neuraminidase for 0 minutes at 37 C.The anodai direction is downward.The more anodal zone is liver phosphatase. B, Densltometric scans of electrophoretic patterns shown in A. Broken line, Scan of mixture of untreated sera solid line, scan of mixture of sera treated briefly with neuraminidase. The anode is to the left. (From Moss DW, Edwords RK. In proved electrophoretic resolution of bone and liver alkaline phosphatases resulting from partial digestion, with neuraminidose. Clin Chim Acta 1984 143 i 77-82.) ...

Jeffers (T2) did electrophoretic studies on serum alkaline phosphatase in hepatobiliary and skeletal disease (Fig. 1). They found eight separate zones of alkaline phosphatase activity arranged in three distinct patterns, and suggested the use of the starch-gel zymogram in the differentiation between obstructive, metastatic, or infiltrative hepatobiliary disease, parenchymal hepatic disease, and osteoblastic disorders. 

E12. Evans, D. A. P., Confirmation of association between ABO blood groups and salivary ABH secretor phenotypes and electrophoretic patterns of serum alkaline phosphatase. J. Med. Genet. 2, 126-127 (1965). 

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