Electrophoretic mobility in gels

The electrophoretic mobilities in gels are generally related to the mobilities in free solution by the empirical logarithmic Ferguson equation [18,115,154]... 

Rodbard and Chrambach [77,329] developed a computer program that allows the determination of molecular parameters, i.e., free mobility, molecular radii, molecular weight, and charge or valence, from measured electrophoretic mobilities in gels with different monomer concentrations. For a set of mobility versus gel concentration data they used the Ferguson [18,115,154] equation to obtain the retardation constant from the negative slope and the free mobility from the extrapolated intercept. From the retardation constant they determined the molecular radius using... 

In defining product variants, separation of the product based on physiochemical properties (e.g., charge heterogeneity profiling on ion-exchange chromatography, electrophoretic mobility in gel/CE, or hydrodynamic size distribution in SEC) must be done. In each of these instances, quantitative and sensitive analytical methods using various separation modes of HPLC, gel, and CE are applied to identify and characterize product-related variants. 

The ADSA Protein Nomenclature Committee (Eigel et al. 1984) recommends that these minor K-caseins be identified temporarily according to their genetic variant and numbered consecutively in their order of increasing relative electrophoretic mobility in alkaline urea gels in the presence of mercaptoethanol (Yaguchi et al 1968) as K-casein A-l or K-casein B-l, etc. 

Its relative electrophoretic mobility in denaturing and non-denaturing polyacrylamide gels indicated that it had a dimeric structure with an estimated molecular mass of 65kDa per monomer (Figure 11.1). This was confirmed by... 

Table 20-3 lists the principal plasma proteins and their half-lives, pi, molecular weights, and preferred method of analysis the individual proteins are listed in the order of their electrophoretic mobilities in agarose gels at pH 8.6. These proteins are described later in this chapter other chapters in this book describe many more proteins enzymes (see Chapter 21) lipoproteins (see Chapter 26) hormones (see Chapter 28) and hemoglobin, fibrinogen, and other coagulation proteins (see Chapter 31). The interim consensus reference intervals for 14 plasma proteins are listed in Table 20-4, pending the publication of more definitive intervals. . 

The PS I polypeptides of barley have been named using the nomenclature of Schantz and Bogorad (7). Apparent molecular masses are based on electrophoretic mobilities in SDS-polyacrylamide gels (3,4,5). Calculated molecular masses are based on amino acid or nucleotide sequencing data and on plasma desorption mass spectrometry measurements (27,28). A capital P indicates the location of the gene in the nuclear genome. 

However, since the length of the poly(A) tracts isolated from the different viral RHA structures were deduced essentially from their electrophoretic mobility in polyacrylamide gels under non-denaturing conditions, and there is no report on the ratios of internal AMP to 3 terminal adenosine residues of the "longer" poly(a) tails, it is not yet possible to exclude that the reported difference in length might actually result from hybridization of the poly(a) to other ERA fragments. 

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