Wall coating, electrophoresis

Pai, Y. E, Chun-Chi Lin, C. C., and Liu, C. Y. (2004). Optimization of sample stacking for the simultaneous determination of nonsteroidal anti-inflammatory drugs with a wall-coated histidine capillary column. Electrophoresis 25, 569—577. 

Vindevogel, J., and Sandra, P. (1994). On the possibility of performing chiral wall-coated open-tubular electrochromatography in 50 pm internal diameter capillaries. Electrophoresis 15, 842-847. 

Timerbaev, A.R. Buchberger, W. Prospects for detection and sensitivity enhancement of inorganic ions in capillary electrophoresis. J. Chromatogr., A 1999, 834, 117-132. Horvath, J. Dolnike, V. Polymer wall coatings for capillary electrophoresis. Electrophoresis 2001, 22, 644-655. 

Horvath J, Dolnik V (2001). Polymer wall coatings for capillary electrophoresis. Electrophoresis. 22 644-655. 

Dolnik, V. Wall coating for capillary electrophoresis on microchips. Electrophoresis, 25, 3589-3601, 2004. 

Shah, J.J., Geist, J., Locascio, L.E., Gaitan, M., Rao, M.V., and Vreeland, W.N. (2006) Surface modification of poIy(methyI methacrylate) for improved adsorption of wall coating polymers for microchip electrophoresis. Electrophoresis, 27 (19), 3788 -3796. 

J. E. Melanson, N. E. Baryla, C. A. Lucy, Double-chained surfactants for semipermanent wall coatings in capillary electrophoresis. Anal. Chem., 2000, 72, 4110-A114. 

Horvath, J. Dolnike, V. Polymer wall coating for capillary electrophoresis. Electrophoresis 2001, 22, 644-655. 

Capillary zone electrophoresis also can be accomplished without an electroosmotic flow by coating the capillary s walls with a nonionic reagent. In the absence of electroosmotic flow only cations migrate from the anode to the cathode. Anions elute into the source reservoir while neutral species remain stationary. 

A number of developments have increased the importance of capillary electrophoretic methods relative to pumped column methods in analysis. Interactions of analytes with the capillary wall are better understood, inspiring the development of means to minimize wall effects. Capillary electrophoresis (CE) has been standardized to the point of being useful as a routine technique. Incremental improvements in column coating techniques, buffer preparation, and injection techniques, combined with substantive advances in miniaturization and detection have potentiated rugged operation and high capacity massive parallelism in analysis. 

Three bacterial species (E. coli, P. putida, and S. rubidae) were separated on isoelectric focusing in methylcellulose coated capillaries, and three bacterial species (P. fluorescens, E. aerogenes, and M. luteus) and the yeast S. cerevisae, were separated by capillary electrophoresis in the presence of polyethylene oxide.101 The polymers served to minimize adsorption to the walls without causing cellular lysis. 

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