Electrophoresis isotachophoresis

There are a few other analytical methods in which electrochemistry plays an essential role, such as (paper) electrophoresis, isotachophoresis, electrography and electrochromatography (according to Fujinaga) as they belong to analytical separation techniques, they are beyond the scope of this book. 

The development of electrophoretic techniques afforded possibilities for fractionations based on charge density differences. Duxbury (1989) has reviewed applications of different electrophoretic separation methods, including zone electrophoresis, moving boundary electrophoresis, isotachophoresis, and isoelectric focusing (IEF). Preparative column electrophoresis (Clapp, 1957) and continuous flow paper electrophoresis (Hayes, 1960 summarized by Hayes et al., 1985) methods have been used to separate components isolated from sapric histosol soils. These techniques allowed separation of polysaccharides from the colored components the electrophoretograms of the colored components were diffuse, showing a continuum of components of different charge densities. 

Bodor, R., Kaniansky, D., Masdr, M., Silleova, K., Stanislawski, B., Determination of bromate in drinking water by zone electrophoresis-isotachophoresis on a column-coupling chip with conductivity detection. Electrophoresis 2002, 23, 3630-3637. 

CE combines the classic techniques of zone electrophoresis, isotachophoresis, isoelectric focusing, and gel electrophore-... 

The world of electromigration separations is sharply divided into two areas. Zone electrophoresis on paper and related procedures have (in spite of their wide applicability to diverse organic compounds) already passed their period of favour. The other branch is represented by the more recent techniques some of which have already became widely accepted (such as isoelectric focusing or separations in polyacrylamide gel) and the others that are at the moment in the centre of a rapid development like displacement electrophoresis (isotachophoresis). This chapter is devoted mainly to analytical procedures such as these which are governing the area of electromigration separations at the moment with a single exception flow deviation (curtain) electrophoresis which will be discussed in more detail because it offers several new dimensions in the separation field. The other preparative procedures are summarized only briefly. 

Electrically charged compounds can be transported in a gel or liquid under the influence of an electric field and this process has been termed electrophoresis. There are basically three different electrophoretic methods of separation and they are, zone electrophoresis, isotachophoresis and isoelectric focusing. 

See also Carbohydrates Overview. Electrophoresis Isotachophoresis Isoelectric Focusing. Laser-Based Techniques. Liquid Chromatography Overview Size Exclusion. 

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