High performance capillary electrophoresis isotachophoresis

Finally, when RPC methods are used in preparative studies with peptides, the opportunity routinely exists for subsequent analysis of the recovered fractions by a variety of analytical methods including high-speed RP-HPLC, HP-IEX, HP-HILIC, or HP-IMAC, zonal or micellar electrokinetic high-performance capillary electrophoresis (HP-CZE and MECK-CZE), capillary electrochromatography (CEC), or capillary isotachophoresis. The combination of the RPC information, drawn from the In k versus i > plots, with the data derived from on-line spectroscopic detection thus readily provides a comprehensive opportunity to assess the purity of an isolated peptide, many of the physicochemical features of the interaction, as well as a means to optimize the resolution in the RPC separation. 

Gysler, J., Mazereeuw, M., Helk, B., Heitzmann, M., Jaehde, U., Schunack, W., Tjaden, U.R., and van der Greef, J., Utility of isotachophoresis-capillary zone electrophoresis, mass spectrometry and high-performance size-exclusion chromatography for monitoring of interleukin-6 dimer formation, /. Chromatogr. A, 841, 63, 1999. 

Triethanolamine can be determined in metalworking and cutting fluids by gas chromatography-mass selective detection of silylated derivatives, by isotachophoresis, by capillary zone electrophoresis with indirect ultraviolet detection, and by spectrophotometry (Kenyon et al, 1993 Fernando, 1995 Schubert et al, 1996 Sollenberg, 1997) and in cosmetics and pharmaceuticals by ion-exclusion chromatography and by reversed-phase high performance liquid chromatography (Fukui et al, 1992 Maurer etal, 1996). 

A. J. Tomlinson and S. Naylor, Enhanced performance membrane preconcentration-capillary electrophoresis-mass spectrometry (mPC-CE-MS) in conjunction with transient isotachophoresis for analysis of peptide mixtures,./. High Resolut. Chromatogr. 18 384-386(1995). 

Ackermans, M.T. Beckers, J.L. Everaerts, F.M. Seelen, I.G. Comparison of isotachophoresis, capillary zone electrophoresis and high-performance liquid chromatography for the determination of salbutamol, terbutaline sulphate and fenoterol hydrobromide in pharmaceutical dosage forms. J.Chromatogr, 1992, 590, 341-353... 

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