Cryo-electron tomography

Kurner, J., Frangakis, A.S. and Baumeister, W. (2005). Cryo-electron tomography reveals the... 

Cryo-electron tomography + + + Cellular interactome Nickell et al. (2006)... 

Recently a number of publications has demonstrated the feasibility of cryo-electron tomography (using an energy filter) to investigate larger, intact structures, such as the complete Herpes Simplex Virus (see Figure 2.12) and a small eukaryotic cell. 

Bongini, L., Fanelli, D., Piazza, R, et al. (2005) Dynamics of antibodies from cryo-electron tomography. Biophys Chem, 115 (2-3), 235-240. 

Stoffler, D., B. Feja, B. Fahrenkrog, J. Walz, D. Typke and U. Aebi (2003). "Cryo-electron tomography provides novel insights into nuclear pore architecture Implications for nucleocytc lasmic transport" 7 3/0/Bio/328(1) 119-30. 

Figure 10 Components of the nuclear pore complex. Structures are available from the PDB and EMDB for some of the components of the nuclear core complex. The individual proteins and small subassemblies shown in ribbon representation are from six PDB entries that provide atomic-level information from X-ray (4GQ2, 3UKY, 4FHN, 3TKN, 4GQ1) or nuclear magnetic resonance spectroscopy (2EC1) studies. The three larger subassemblies drawn as surfaces have been analyzed by cryo-electron microscopy (EMD-5152, EMD-1097) or cryo-electron tomography (EMD-1394).

M.Beck, V.Lucic, F.Forster, W.Baumeister,and O.Medalia,Nat rc,449,611—615(2007). Snapshots of Nuclear Pore Complexes in Action Captured by Cryo-Electron Tomography. 

Structure, M, and shape Static light scattering, dynamic light scattering, sedimentation measurements, small-angle X-ray, solution viscosity, imaging methods in situ) AFM, cryo (HR-)TEM, electron tomography)... 

Objective lens and specimen stage is the heart of a transmission electron microscope. Modem objective lenses are a twin lens instead of a single lens. It has an upper and a lower pole-piece with a gap in between. The resolution of a microscope is directly determined by the pole-piece gap. Small pole-piece gaps allow high resolution, but limit tilt angles. A larger pole piece gap reduces resolution but allows other TEM applications such as tomography, cryo TEM, environmental TEM, and dynamic experiments. 

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