EGFP, effect

Figure 2.23 Combined transmission and fluorescence (the inset shows the spectrum of EGFP taken from cell cytoplasm) spectra show that cells are effectively transfected with pEGFP when delivered with ORMOSIL nanoparticles. (Reproduced from Ref. 17, with permission.)...

Fig. 6. Effect of a 13.5-h cis- or trans -DDP treatment on doxycycline-inducible EGFP expression in HeLa Tet-on cells. The fluorescence values were divided by total protein to correct for variations in cell count.

Howard and Kjems (2006) reported that chitosan/siRNA complexes effectively silenced EGFP expression in both H1299 human lung carcinoma cells (77.9% reduction) and murine peritoneal macrophages (89.3% reduction). Katas and Alpar (2006) reported that preparation methods such as... 

Fig. 3. Effects of AMAP2 overexpression on receptor internalizations in HeLa cells. Clathrin-independent endocytosis of Tac molecules (A and C) and clathrin-dependent endocytosis of Tfn molecules (B and D) were analyzed. See text for details., P<0.01 against values of the EGFP control in (A) and (B). In (C), cells marked by asterisks expressed detectable levels of EGFP-AMAP2 or dynamin2 K44A, but exhibited more than 20% of Tac uptake as compared to those of the untransfected cells in the same field. These cells were hence judged as cells in which Tac uptake was not blocked. Panels A an B are reproduced from Hashimoto et al. (2004b).

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