Effect of Temperature on Separation Efficiency

One of the reported advantages of raising the temperature of a chromatographic separation is an increase in peak efficiency. This is usually attributed 

In such a situation the internal diameter of the column might also effect the equilibration process but Molander et al. [37] found that even using a temperature gradient, the differences were minimal for columns narrower than 4.6 mm internal diameter. A recent study has found that elevated temperatures, up to 70°C, markedly improved the efficiency and peak shapes of bases with intermediate pH eluents [38]. 

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