Effect of Temperature on Enzyme Stability and Activity

Reciprocal plots of Ifv versus 1/[S ] at different fixed concentrations will yield different values of from which K, (hence, pK.) can be obtained in the usual way. 

The relationship between the rate constant of a reaction, k, and the activation energy, K, is given by the Arrhenius equation (Chapter 3)  

A is a constant for the particular reaction. A plot of log k versus 1/T is linear (Fig. 4-39). The integrated form of the Arrhenius equation is  

In a simple rapid equilibrium system, = kp, a. first order rate 

The effect of temperature on the rate of reactions is frequently expressed in terms of a temperature coefficient, Qio, which is the factor by which the rate increases by raising the temperature 0 C. 

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