Effect of sucrose on the bimolecular diffusion constant

We have simplified the analysis to two domains, the whole protein (Tq =17.8 ns) and a more restricted area, the heme pocket and some of the protein matrix surrormding it (To = 10 ns at 68 niM of oxygen at 20°C and 8.3 ns at 53 niM of oxygen at 35°C) (Only, plots at 20°C are shown, figure 9.20). From the slopes at low oxygen concentrations, we found values of kq equal to 8.4 x 10 M s and 13 x 10 M s at 20 and 35°C, respectively. The slopes at high quencher concentrations give values of kq equal to 3.65 

Fluorescence quenching with oxygen of was also studied by separating the 

FLUORESCENCE FINGERPRINTS OF ANIMAL AND VEGETAL SPECIES AND / OR VARIETIES 

Fluorescence fingerprints of Eisenia fetida and Eisenia andrei 

NMR studies performed on tissue extracts on the coelomic fluid of Eisenia andrei and Eisenia fetida allowed to distinguish the two species one from each otlier (Bmidy et al. 2002). We have seen in all over this book that fluorescence spectroscopy can detect differences that occur on the structural and dynamical levels purified macromolecules. The following data will show that it is possible to use fluorescence spectroscopy to detect differences in the cells at the structural and the metabolical levels. 

---