Effect of Nitrile on the AMase Activity and Stability

On the contrary, it has to be pointed out that very high concentrations of N-heterocycHc nitriles (up to 12 M) had been transformed to the corresponding amides via NHase-catalyzed reactions. Moreover, the NHase activity of whole cells of R. rhododirous PA-34 was used for its high butyronitrile tolerance and utiHzed for butyramide production [32]. 

A previous study, aiming to reach a high conversion of 3-cyanopyridine into nicotinic acid, showed that by adopting two CSMRs arranged in series, the rate of 

The irreversibility of the inactivation was examined by feeding the reactor with a buffered solution of 100 mM nicotinamide from 158 h run time onwards, and, as expected, the AMase activity was not restored in any of the runs. 

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