Effects of Enzyme Concentration

Guraya, H. S., James, C., Champagne, E. T. (2001). Effect of enzyme concentration and storage temperature on the formation of slowly digestible starch from cooked debranched rice starch. Starch/Starke, 53, 131-139. 

Figure C4.1.3 Range-finding and the effect of enzyme concentration on the time course of a reaction. The dotted lines show the true initial rate. Only assay C will be reliable if a fixed-time assay is used.

To measure the activity of an enzyme of the citric acid cycle, isocitrate dehydrogenase, and the effect of enzyme concentration on the rate of reaction. 

Sattler, W., Esterbauer, H., and Glatter, Steiner, O., The effect of enzyme concentration on the rate of the hydrolysis of cellulose. Biotech. Bioeng. Symp. 1989, 33, 1221-1234. 

Figure 1. Effect of enzyme concentration on freeze-thaw inactivation of catalase. Catalase was frozen unseeded at 15°C/min to —78° and warmed at...

Figure 7.15 Effect of enzyme concentration on conversion and capacity of a UF reactor. Pronase-Promine D at pH 8.0, 50°C.19...

Figure 1. Effect of enzyme concentration and of temperature on hydrolysis of carboxymethylcellulose and filter paper by T. viride cellulase...

Figure 8. Effect of enzyme concentration on extensive hydrolysis of cellulose by T. viride cellulose...

Enzyme Studies. (1) Effect of Temperature and pH on the Inactivation of Cellulase. Details of experiments and results on the effects of temperature on the inactivation and adsorption of Tv cellulase, effect of pH on adsorption, activity, and inactivation of To cellulase, effect of enzyme concentration on saccharification of cellulose, and glucose inhibition of saccharification of cellulose have been reported in a separate communication (3). These data show that small enzyme losses (up to 10%) are difficult to detect. Tv cellulase alone does not appear to lose... 

Thus, the availability of more enzyme molecules to catalyze a reaction leads to the formation of more ES and a higher reaction rate. The effect of enzyme concentration on the rate of a reaction is shown in I Eigure 10.5. As the figure indicates, the rate of a reaction is directly proportional to the concentration of the enzyme—that is, if the enzyme concentration is doubled, the rate of conversion of substrate to product is also doubled. 

FIGURE 5 Effect of enzyme concentration (gm) on beads catalytic activity. 

Paolucci-Jeanjean D, Belleville M P, Rios G M, Zakhia N (2000), The effect of enzyme concentration and space time on the performance of a continuous recycle membrane reactor for one-step starch hydrolysis, Biochem. Eng. J., 5, 17-22. 

Effect of Enzyme Concentration on Af-AcETYL Cysteine Formation at pH... 

The response characteristics of the ERM are, of course, a vital property of the material. The response time of the material to the stimulus includes important parameters such as the lag time between enzyme reaction and material response, the effect of enzyme concentration on the material response and the duration of the material response. These properties are most frequently studied in systems that are designed for the dehvery of drugs as the release kinetics of drugs are vital for treatment (McDonald et al., 2009 Itoh et al., 2006 Koga et al., 2011 Patrick and Ulijn, 2010 Wang et al., 2010 Weinstain et al., 2009). In many ERMs the response time is actually rather slow compared to other stimuh-responsive mechanisms and can take several hours to days to reach completion. 

FIg. 7. Effect of enzyme concentration on the maximal velocity (F) of the reaction measured at high substrate concentration. 

Figure 2.2a shows the effect of enzyme concentration (1.5-5%) on the H2S removal from simulated biogas for a period of 8h. Yellow line represents the H2S concentration in the feed stream (3009 ppm). H2S in the outlet gas was detected only for enzyme concentrations of <4%. H2S concentration lines show a breakthrough that depends on the enzyme concentration. 

Effect of enzyme concentration — Enzymatic inhibition occurs via a well-defined chemical coupling. The rate of the enzyme-catalysed reaction is then a function of the concentration of the enzyme with respect to that of the inhibitor. As the enzyme concentration increases, the inhibitor concentration must also increase to ensure the same degree of inhibition (Figure 4.12). 

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