EDC coupling

As an example, Nakayama et al. thermally oxidized CNTs to introduce carboxyl groups at the opened CNT tips and then covalently linked ethylenediamine as a crosslinker (via EDC coupling) to provide primary amino groups that formed peptide bonds with N-hydroxysuccinimide-functionalized proteins (Fig. 5.2) [26]. 

EDC-mediated coupling This step is crucial for the success of the immobilization procedure. EDC reacts with the carboxyl groups to form an amine-reactive O-acylisourea intermediate. If this intermediate does not encounter an amine, it will hydrolyze and regenerate the carboxyl group. In the presence of N-hydroxysulfosuccinimide (Sulfo-NHS), efficiency of EDC coupling is increased by the formation of an amine-reactive Sulfo-NHS ester intermediate, which has... 

With the purpose of conferring thermosensitivity to chitosan-based hydrogels. Park et al. proposed the grafting of carboxylic acid-terminated poly(ethylene oxide-h-propylene oxide) block copolymer (Pluronic) onto the primary amine of chitosan, mediated by EDC coupling agent [102]. With the same purpose, Wang et al. grafted poly(N-isopropyl acrylamide) (NiPAM) chains onto chitosan by the copolymerization of acrylic acid-derivatized chitosan and N-isopropylacrylamide (NIPAAm) in aqueous solution [103]. 

PEG-diacylchloride (COCl-PEG-COCl) was attached to ultra-fine cellulose fiber surfaces via ester bond formation between the COCl of the PEG-diacylchloride and the OH on the cellulose (Scheme 6). The quantities of COCl or COOH could be optimized by varying the COCl/OH molar ratio and PEG chain length. Based on the quantification of COOH by the silver o-nitrophenolate method (27), the PEG attached cellulose fibers contained up to 1.0 mmol of COOH per g of cellulose unless specified otherwise. Lipase was covalently bound with the PEG-attached cellulose fibers in pH 4 buffer in the presence of a carbodiimide (EDC) coupling agent and incubated for 7 hrs. Lipase bound fibers were then rinsed in buffers of increasing pH of 4, 7, and 10, then dried under vacuum at ambient temperature for 12 hrs. 

For our specific synthesis, the allyl ester substrate for the Ireland-Claisen 1 was obtained in a 78% yield through a l-ethyl-3-(3-dimethylaniinopropyl) carbodiimide (EDC) coupling reaction between a trifluoroacetyl-protected glycine and allyl alcohol (Scheme 2). Reaction conditions discovered by Kazmaier were then used to perform the Ireland-Claisen reaction and... 

Tertiary butyl hydrazone nadic carboxylate was prepared by coupling of nadic acid and tertiary butyl carbazate by using 1.2 equivalent of N-(3-dimethylaminopropyl)-N -ethylcarbodiimide hydrochloride (EDC) coupling reagent in presence of 1.2 equivalent of 1-hydroxybenzotriazole (HOBT) in dimethyl formamide solvent. Formation of monomer that was thoroughly characterized by H NMR and IR spectroscopy. The signal 5... 

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