Dynamic reserve

A simple alternative to direct detection of intrinsic protein fluorescence detection is the technique of indirect fluorescence detection proposed by Kuhr and Yeung.20 In this approach, the analysis buffer contains a fluorescent anion that produces a high background fluorescence signal. Nonfluorescent analyte anions displace the fluorescent species, producing a zone of reduced signal. Sensitivity in indirect fluorescence detection is determined by the dynamic reserve (ratio of signal... 

Noise consists mainly of two elements instrumental noise, which includes the lamp and its age, and noise due to the high background absorptivity. As this is an important parameter, Yeung has proposed the term the dynamic reserve (D ) for this ... 

From Equation (3), we learn that increasing the absorptivity of the probe and the detector pathlength will lower the LOD, while the noise should be as low as possible. However, some of these factors are interdependent lowering the concentration of the probe will decrease the dynamic reserve and increasing the detector pathlength will increase the baseline noise. 

To optimize sensitivity in indirect detection, the concentration of the mobile phase additive should be kept as low as possible and the ratio of background signal to background noise (dynamic reserve) should be as large as possible. Another important factor is the transfer ratio, the number of molecules of the background buffer additive that are displaced by a molecule of analyte. For the best sensitivity, this ratio should be large. 

Very wide dynamic range. Usually a transfer characteristic curve (input-to-output) with a slope ( ) of 1 is ideal. However, a capability to alter this slope electronically can be advantageous when very high dynamic reserves are necessary with pixels of limited capacitance. 

Suppiy chain Buffer inventories Excess capacity Alternative suppliers Recovery plans - select scenarios Co-ordinated forecasts throughout supply chain What if modelling Agility products and processes Supply chain transparency War gaming Dynamic reserves of critical components... 

This technique has permitted the dynamics of the exo-cytic/endocytic cycle to be investigated. At the neuromuscular junction, a readily releasable pool (RRP) and a reserve pool of vesicles coexist, the latter being released... 

If the values for all the factors are known and expressed in common units, this equation will provide a reasonable estimate of flow from recovery wells. The difficulty of actual application of this equation is the determination of the water-oil saturation in the aquifer matrix (thus, the relative permeability). The equation assumes a steady-state flow setting. In a dynamic situation where the oil reserves are being depleted and water-oil mixtures are variable, it is almost impossible (from an economic point of view) to use these equations precisely. However, inclusion of some assumptions based on results of the initial site investigation can often be of assistance in initial spacing of recovery wells and estimating recovery rates. 

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