Dynamic photobleaching

Bopp M A, Jia Y, Li L, Cogdell R J and Hochstrasser R M 1997 Fluorescence and photobleaching dynamics of single light-harvesting complexes Proc. Natl Acad. Sc/. USA 94 10 630-5... 

Rabut, Q., and Ellenberg, J. (2005). Photobleaching Techniques to Study Mobility and Molecular Dynamics ofProteins in Living Cells FRAP, iFRAP, and FLIP. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. 

White, J., and Stelzer, E. (1999). Photobleaching GFP reveals protein dynamics inside live cells. Trends Cell Biol. 9, 61-65. 

T. P. Burghardt and D. Axelrod, Total internal reflection/fluorescence photobleaching recovery study of serum albumin adsorption dynamics, Biophys. J. 33, 455-468 (1981). 

Luminescence is often much more sensitive to molecular dynamics than other optical techniques where temperature, viscosity, pH and solvent effects can have a significant influence on the emission response. Analyte degradation for light sensitive fluors and photobleaching for static measurements also influence the emission signal. Because of the wide variety of potential matrix effects, a thorough investigation should be conducted or the sample matrix well understood in terms of its potential impact on emission response. A complete discussion on the fate of the excited states and other measurement risk considerations can be found elsewhere. ... 

Yu and Russo 52a reported the fluorescence photobleaching recovery and dynamic light... 

The fluorescent spectroscopy is one of the optical techniques that are widely used in the study of structure and dynamic properties of lipids in lamellar phases [76]. The Fluorescence Recovery after Photobleaching (FRAP) is successfully applied in the lateral diffusion studies of BLM (e.g. 77]. FRAP has been employed to study similar phenomena at the air/water interface of Langmuir trough [78]. 

Consider surfaces that are inert and may be made (molecularly) smooth, so that, optically speaking, they may be treated as Fresnel surfaces. Mica, certain polished glasses, quartz and silicon wafer surfaces may belong to this category. For such well-defined systems the optical techniques introduced in sec. 1.7.10 come to mind reflectometry, ellipsometry, and (to study the dynamics) fluorescence recovery after photobleaching (FRAP). The principles of these techniques have been outlined in that section. 

Edidin M. Huorescence photobleaching and recovery, FPR, in the analysis of membrane structure and dynamics. In Mobility and Proximity in Biological Membranes. Damjanovich S, Edidin M, Szollosi J, Tron L, Eds. 1994. CRC Press, Boca Raton, FL. 

Thompson NL, Steele BL. Total internal reflection with fluorescence correlation spectroscopy. Nat. Protoc. 2007 2 878-890. Sund SE, Axelrod D. Actin dynamics at the living cell submembrane imaged by total internal reflection fluorescence photobleach-ing. Biophys. J. 2000 79 1655-1669. 

Lippincott-Schwartz J, Altan-Bonnet N, Patterson G. Photobleaching and photoactivation following protein dynamics in living cells. Nat. Cell Biol. 2003 7-14. 

E.L. Elson. 1986. Membrane dynamics studied by fluorescence correlation spectroscopy and photobleaching recovery... 

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