Affinity chromatography dye-ligand

Cibacron Blue 3GA, for dye-ligand affinity chromatography, 6 402 Cibacron C dyes, 9 475 Ciba Specialty Chemicals, 19 448 Cichlids, world aquaculture production in 1996, 3 186t... 

Dyeing applications, tetrahydrofurfuryl alcohol in, 12 279 Dyeing theory, 26 394-395 Dyeing transition temperature, 9 159 Dye intermediates, 9 265-298 chemistry, 9 266-291 classification, 9 265-266 economic aspects, 9 293-295 equipment and manufacture, 9 291-293 health and safety factors, 9 295-298 obtained by nitration, 9 2 7 It obtained by sulfonation, 23 525 unit processes, 9 269-283 Dye lasers, 74 702-705 23 144 output characteristics of, 74 705 Dye-ligand affinity chromatography, 6 402 Dye liquor, 9 163 Dye manufacturing... 

Procion Rubine MX-B, Procion Yellow H-A, and Turquoise MX-G. These dyes have found use over the last few decades in the purification of a broad range of proteins and enzymes, including albumin, decarboxylases, glycolytic enzymes, hydrolases, lyases, nucleases, oxidoreductases, synthetases, and transferases [77,78], The first use of dye-ligand affinity chromatography was described by Staal et al. in 1971 [79], Since that time, it has become an extremely popular tool for enzyme and protein purification, with hundreds of such compounds having been isolated by this technique [3-6,76-79],... 

Worrall, D.M. (1996) Dye-ligand affinity chromatography, Methods Mol. Biol. 59,169-176. 

Figure 2 Structure of Cibacron Blue 3G-A, a stationary phase often used in dye-ligand affinity chromatography.

Fig. 3 Cibacron Blue 3GA, a triazine dye that is commonly used as a ligand for protein and enzyme purification in the method of dye-ligand affinity chromatography.

It is worth mentioning that dye-ligand affinity chromatography has found application within a relatively short time in the isolation and purification of a series of dehydrogenases, phosphotransferases, t-RNA synthetases, restriction nucleases, initiation factors of protein biosynthesis, a-fetoprotein, clotting factors II and X and complement factors. 

Labrou NE. Dye-ligand affinity chromatography for protein separation and purification. In Walker JM, editor. Methods in molecular biology, affinity chromatography methods and protocols. Totowa, NJ Humana Press 2000. p. 129-39. 

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