Dulbecco’ phosphate-buffered saline

The specific protocol used by Osiris for OTI-010 isolation and expansion was outlined in the paper by Aggarwal Pittenger [643694]. Briefly, BM aspirates (10 ml) were combined with Dulbecco phosphate-buffered saline (DPBS, 40 ml) and centrifuged at 900 g for 10 min at 20°C. The cells were then resuspended and gently layered onto a Percoll cushion (density =... 

To establish a good in vitro/in vivo correlation, the composition and ionic strength of the receptor solution must be adjusted. For fentanyl, the best correlation was obtained with a receptor solution comprised of a 10-fold dilution of modified Dulbecco phosphate-buffered saline (without calcium or magnesium) at pH 7.4. The currents tested in vitro ranged from 0 to 400 pA and were applied to a silver anode and a silver chloride cathode using a constant current power supply. The in vitro data presented in Fig. 5 clearly demonstrate the expected linear dependence of fentanyl delivery on current and the excellent correlation to the in vivo data. 

Males were anesthetised and mental glands (N 200) were surgically removed. Secreted components were extracted into 0.8 mM acetylcholine chloride in l/2x PBS (cf Rollmann, Houck and Feldhoff 1999). Gland extracts were centrifuged for 10 min at 14,000 g and the supernatant was removed. The supernatant was filtered (0.2 pm) and loaded as aliquots onto a Sephadex Superfine G-75 gel filtration column (1.6 cm x 15.5 cm Pharmacia, Piscataway, NJ) on a Waters HPLC system (Mil-lipore, Milford, MA). The column had previously been equilibrated with one-half strength Dulbecco s phosphate buffered saline (l/2x PBS). The column was eluted... 

Dulbecco s modified phosphate-buffered saline (PBS) without calcium chloride and magnesium chloride (Pierce Biotechnology, Rockford, IL). 

Dulbecco s phosphate-buffered saline (PBS) (with calcium and magnesium) (GIBCO/BRL, Grand Island, NY). 

Dulbecco s phosphate-buffered saline (PBS) 100 mg anhydrous calcium chloride, 200 mg potassium chloride, 200 mg monobasic potassium phosphate, 100 mg magnesium chloride 6 H2O, 8 g sodium chloride, and 2.16 g dibasic sodium phosphate 7 H2O bring volume to 1 L with deionized glass-distilled water, pH 7.4. 

Bovine serum albumin (BSA) solution essentially fatty acid free (MW = 66,200 Sigma A-6003) To make 5 ml BSA solution, 0.9 g BSA is added to a 50 ml conical tube containing 5 ml phosphate-buffered saline (PBS Dulbecco s 1 x PBS, Gibco Laboratories, 14190-144). 

Primary fibroblasts from skin biopsy samples are grown at 37°C in the presence of 5% C02 in Dulbecco s modified Eagle s medium (DMEM) supplemented with 10% foetal calf serum (FCS) and 1% penicillin/streptomycin. For passaging, confluent cells are washed with phosphate-buffered saline (PBS) and incubated for 5-10 min at 37°C in the presence of trypsin. 

Dulbecco s phosphate-buffered saline (DPBS), pH 7.2 (Hyclone SH30013)... 

Con A, twice crystallized in saturated sodium chloride solution, was obtained from Miles Laboratories (Elkhart, Ind.). Fragmented sub-units were removed by gel filtration on Bio-Gel P-100 (11). Solutions were made up in Dulbecco s phosphate buffered saline (PBS) pH 7.4, and the concentration routinely determined by UV spectrophotemetric analysis based on (1 cm path) = 13.0. 

Dulbecco s phosphate buffered saline (PBS) is similar to Hanks BSS but bicarbonate is omitted and the levesl of Na2HP04 and KH2P04 are raised to provide increased buffering capacity. It is not used as a basis for growth medium but is often used for washing cell monolayers. PBS is made up in three parts (Appendix 1 Table 2). PBS solution A (PBS-A) lacks the Ca2+ and Mg2 + ions which are... 

Dulbecco s Phosphate Buffered Saline (D-PBS) (lx) (Gibco) for cell washing. 

Buffer Dulbecco s Ca +-Mg +-free phosphate-buffered saline (Grand Island Biological Co., Grand Island, New York) (PBS) Standard lipids chromatographically pure phosphatidylcholine, tri-palmitin, palmitic acid, linoleic acid Silica gel chromatography plates, 0.25 mm (EM Laboratories, Elmsford, New York)... 

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