Drug bioanalysis chromatography

An application of molecular imprinting in drug bioanalysis closer to practical realisation is probably that of solid phase extraction (SPE). This application does not suffer to the same extent from the drawbacks generally associated with MIPs, such as peak broadening in liquid chromatography and leakage of imprint mole-... 

Drug bioanalysis Diogoxin Plasma Fluorescence-labeled antibodies, affinity chromatography 0.2 nmol/L 8... 

Andersson, L.I. (2000) Molecular imprinting for drug bioanalysis. A review on the application of imprinted polymers to solid phase extraction and binding assay. Journal of Chromatography B, 739, 163 173. 

Hsieh S. et al., 2004. Increased throughput of parallel online extraction liquid chromatography /electrospray ionization tandem mass spectrometry system for GLP quantitative bioanalysis in drug development. Rapid Commun Mass Spectrom 18 285. 

Goodwin, L., White, S. A., and Spooner, N. (2007). Evaluation of ultra-performance liquid chromatography in the bioanalysis of small molecule drug candidates in plasma. J. Chromatogr. Sci. 45 298-304. 

Larger, P. J., Breda, M., Lraier, D., Hughes, H., and James, C. A. (2005). Ion-suppression effects in liquid chromatography-tandem mass spectrometry due to a formulation agent, a case study in drug discovery bioanalysis. J. Pharm. Biomed. Anal. 39 206-216. 

C. Albrecht, R. Reichen, J. Visser, D. K. F. Meijer, and W. Thormann, Differentiation between naproxen, naproxen-protein-conjugates and naproxen-lysine in plasma via micellar electrokinetic capillary chromatography—A new approach in the bioanalysis of drug targeting preparations, Clin. Chem. 43 2083-2090 (1997). 

Stokvis E, Rosing H, Beijnen JH (2005) Liquid chromatography-mass spectrometry for the quantitative bioanalysis of anticancer drugs. Mass Spectrom Rev 24 887-917... 

Because of the serial nature of LC-MS, much of the current discussion has centered on ways to reduce LC-MS/MS cycle time. Often the rate-limiting step for drug-discovery bioanalysis lies in the speed with which methods (sample preparation and chromatography) can be prepared for NCEs. One of the frequently overlooked steps is the need to tune and optimize MS/MS transitions for the various analytes studied. Fortunately, most MS vendors now offer semi- or fully automated procedures to perform this task. The origin for these procedures can be traced to the seminal work of Whalen et al., who published an automated procedure known as AUTOSCAN [106]. Itis possible to establish experimental conditions with this procedure in the flow-injection analysis (FIA) mode for 96 analytes in less than one hour. 

Yu, S. et al., Bioanalysis without sample cleanup or chromatography The evaluation and initial implementation of direct analysis in real time ionization mass spectrometry for the quantification of drugs in biological matrixes, Anal. Chem., 81(1), 193, 2009. 

Turnpenny P, Fraier D, Chassaing C, Duckworth J. Development of a micro-turbulent flow chromatography focus mode method for drug quantitation in discovery bioanalysis. J Chromatogr B Anal Technol Biomed Life Sci 2007 856 131-140. 

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