Droplet counter current chromatography DCCC

In order to obtain sufficient quantities of these phytoecdysones for more detailed biological studies, droplet counter-current chromatography (DCCC) was adapted . DCCC Is an especially efficient method for the preparative separation of polar compounds like the phytoecdysones. Thus with DCCC, while requiring only small volumes of solvent, more than 50 mg of each of the Ajuga phytoecdysones were rapidly and nondestructlvely separated and fully recovered (Fig. 6) from each 500 mg Injection. 

The genus Tabernaemontana (Apocynaceae) is a wellknown source of a variety of indole alkaloids, many of which have pharmacological interest (40). Cell cultures of plants from this genus have been initiated (41) and were found to produce alkaloids. In order to be able to identify the alkaloids an isolation method has been elaborated, using droplet counter current chromatography (DCCC). 

Becker, H., W. C. Hsieh, and C. 0. Verelis, 1981. Droplet counter-current chromatography (DCCC). Erste Erfahrungen mit einem wasserfreien Trennsystem. GTTFachz- Labor. Suppl. Chromatogr, 81 38-40. 

Fig. 3. Separation of Tabernaemontana alkaloids by means of droplet counter current chromatography compared to a thin-layer chromatograhic separation, y-axis Rf value in TLC system CHCl3-MeOH (9 1) on silica plates X-axis separation in DCCC-system (Table 6).

Partition Chromatography. - Applications of rotation locular and droplet counter current chromatography (RLCCC and DCCC) to the separation of natural products such as flavonone and stilbene glycosides and steroidal glycoalkaloids have been reponed. ... 

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