Double-stranded lambda-phage

Double-stranded lambda-phage DNA (48.5 kbp, 570 jug/mL) was supplied fiom Wal J Pure Chemicals Inc., in TE buffer (10 mM Tris, 1 mM EDTA, pH 8). The DNA solution was dUuted to a final coiKentration of 5.7 x 1(7 ng with the TE buffo- including ImM DMSO. To visualize the DNA, we prestained it with dimoic cyanine dye YOYO-1 (Molecular Probes, Inc., adsoption 491 nm, emission 509 nm), at a ratio of 1 dye/5 base pairs. Mica sheets of... 

Bacteriophage lambda (X) is a good example for considering phage infection. In lytic infection, the injected linear double-stranded X DNA first circularizes. It is then transcribed to produce viral proteins needed for viral DNA replication and packaging as well as many molecules of viral capsid proteins. The viral DNA is replicated and the DNA copies are packaged into new phage... 

Lambda phage is closely related to other bacteriophages, and these are called T3, T7, T5, Mu, T2, and T4. All of these phages corcsist of a double-stranded DNA genome, an icosahedral head, a long cylindrical tail, and a variable number of tail filaments. The related filamentous phages are Ml3, fl, and fd. 

The study of the mechanism of assembly of a phage particle was first approached with coliphage T4 making use of the electron microscope and conditional lethal mutants. > Since then, the most studied phages, among those that contain double-stranded DNA, have been T4, lambda and P22. 

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