Domain families sequence interpretation

All such cases with novel domain family assignments are characterized by poor sequence identity and therefore may not be interpreted as bona fide members of the protein domain families concerned. They may be interpreted as remotely related to the family concerned, and hence functional similarity between such H. pylori protein sequences with members of the family concerned may or may not exist. 

Interpretation of results of these studies is still difficult. Results of two-hybrid methods become more useful if they can be coordinated with other approaches. For example, computational methods can predict interactions from genome sequences alone. 11/0 More than 45,000 interactions have been predicted among yeast proteins. Reliable identification of such motifs as DNA-binding domains and Ca2+- binding domains can complement two-hybrid analysis.11 The yeast genome is predicted to contain 162 coiled-coil sequences and at least 213 unique interactions between them.0 Examination of sequences of protein families in the Protein Data Bank (PDB) led to prediction of 8151 interactions of 664 types between protein families in yeast.P... 

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