Dodecyl-p-maltoside

SO4 >F > BrOs > Cl > Br > N03 >1 > CNS. For cations, the order is NH+ > K+ > Na+ > Li+ > K a2 (Schick, 1962a Ray, 1971b). Here, the reason for the order is not clear. Similar anion and cation effects have been observed on the CMC value of w-dodecyl-p-maltoside solutions (Zhang, 1996). 

Zhang, R., Zhang, L., and Somasundaran, P. 2004. Study of mixtures of n-dodecyl-P—maltoside with anionic, cationic, and nonionic surfactant in aqueous solutions using surface tension and fluorescenee techniques. J. Coll. Interfacial Sci. 278 453. 

Bowes, J.M., Stewart, A.C. and Bendall, D.S. 1983b. Purification of photosystem II from Phormidium laminosum using the detergent dodecyl-p-D-maltoside. Biochim. Biophys. Acta, 725.210-219. 

Membrane pellets were thawed, ice cold 1% dodecyl-p-D-maltoside (a non-ionic detergent) in 20 mM Tris-HCl, pH 7.5, 300 mM NaCl, 5 mM imidazole was added. The solution was stirred on ice for 30 min. 

A POE nonionic, by itself, showed very weak adsorption onto positively charged alumina (Somasundaran, 1983), while dodecyl-p-o-maltoside adsorbed strongly (Zhang, 1997), presumably because of the negative charge on the latter. 

Decyl-P-D-glucoside (in 0.1 MNaCl, pH — 9) Decyl-P-D-maltoside (in 0.1 MNaCl, pH — 9) Dodecyl-P-D-maltoside (in 0.1 MNaCl, pH — 9) C6H13(OC2H4)6OH... 

A number of control experiments were performed to establish that the copurification observed between HOS and HAS was not the result of nonspecific interactions (30). In the first experiment, HOS and HAS were expressed in different cells, and the detergent-solubilized cell lysates were then combined and incubated together prior to protein purification. Again, HOS and HAS were found to copurify. In a second experiment, / -dodecyl p-D-maltoside was used to solubilize HOS and HAS instead of the detergent Triton X-100, and this change was found to have no effect on the interaction between HOS and HAS. Because -dodecyl P-D-maltoside has a lower micelle number as well as a lower critical micelle concentration (cmc), it seems unlikely that the copurification observed is an artifact of the two enzymes being trapped in the same micelle. 

Guanidine hydrochloride (GndHCl) (Sigma-Aldrich) tt-Dodecyl-P-D-maltoside (Anatrace)... 

In most of those studies, the PS-I-RC was applied to a DEAE-cellulose and the Triton was exchanged with Dodecyl-P-D-Maltoside as described in (26). Then, about 20-100 pi of the PS-I-RC solution, in a chlorophyll concentration of about 4 mg/ml, were placed in a crystallization dish with 1-5% PEG-6000 or IM KPi as precipitants. The precipitant concentration in the reservoir buffer outside the crystallization wells was 2-3 times that of the concentration in the protein solution. The dishes were well sealed and crystallization was allowed to occur at 4-25X via v r diffusion as described by Michel et al (15). 

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