DNA sequences into plasmid and phage vectors

Cloning DNA sequences into plasmid and phage vectors 

A suitable DNA vehicle (vector, replicon) which can replicate in 

A means of introducing the recombinant DNA into the host cell (transformation or transfection). 

A method for screening or genetic selection of cells which contain and replicate the desired recombinant DNA. 

Detailed reviews describing the varieties of plasmids and phages which are used for cloning and amplifying DNA sequences are found in Methods in Enzymology, (1979), Vol. 68. This whole volume is devoted to techniques used in recombinant DNA. 

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